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超高效液相色谱-串联质谱法同时测定喘舒片中10种中药活性成分

Simultaneous determination of 10 active ingredients in Chuanshu Tablets by ultra high performance liquid chromatography-tandem mass spectrometry
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摘要 建立了超高效液相色谱-串联质谱法同时测定喘舒片中10种中药活性成分的含量。采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm)分离,以甲醇-0.2%(体积分数)乙酸溶液作为流动相进行梯度洗脱。在电喷雾离子源,多反应监测负离子模式下扫描,以外标法定量。10种中药活性成分在各自质量浓度范围内与色谱峰面积线性关系良好,相关系数均不小于0.9993,检出限为0.594~101.711 ng/mL。样品的平均回收率为93.42%~108.62%,测定结果的相对标准偏差为0.5%~6.6%(n=6)。该方法操作简单、灵敏度高、准确性好,可以为喘舒片的质量控制和后续研究提供方法参考和数据支持。 An ultra high performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of 10 active ingredients in Chuanshu tablets.The separation was performed on ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8μm),and gradient elution was performed with methanol-0.2%(volume fraction)acetic acid aqueous solution as mobile phase.It was scanned in the electrospray ionization source under the multi-reaction monitoring negative ion mode and quantified by the external standard method.The linear relationship between the peak area and the concentration of the 10 active components was good within their respective concentration ranges,with correlation coefficients not less than 0.9993,and the detection limits were 0.594-101.711 ng/mL.The average recoveries were 93.42%-108.62%,and the relative standard deviations were 0.5%-6.6%(n=6).The method has the advantages of simple operation,high sensitivity and good accuracy,which can provide method reference and data support for quality control and follow up research of Chuanshu Tablets.
作者 李敏 于姗姗 齐红 张秋红 LI Min;YU Shanshan;QI Hong;ZHANG Qiuhong(Jinan Supervision and Inspection Center for Food and Drug Control,Jinan 250102,China)
出处 《化学分析计量》 CAS 2024年第11期58-62,共5页 Chemical Analysis And Meterage
基金 山东省食品药品监督管理局2022年药品质量安全风险监测项目(2022-2)。
关键词 超高效液相色谱-串联质谱法 喘舒片 中药活性成分 质量控制 ultra high performance liquid chromatography-tandem mass spectrometry Chuanshu Tablets Chinese medicine activated ingredients quality control
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