基于Fe_(3)O_(4)@MnO_(2)纳米粒子类氧化酶活性特异性检测酚类物质

Specific detection of phenolics based on oxidase-like activity of Fe_(3)O_(4)@MnO_(2)nanoparticles

制备了具有高效类氧化酶活性的Fe_(3)O_(4)@MnO_(2)纳米粒子,该粒子具有性质稳定、类氧化酶活性强、制备简单等优势,结合酚类物质的酶抑制作用,构建了一种简单、快捷、现象明显的温度检测方法,弥补了传统检测方法的不足。Fe_(3)O_(4)@MnO_(2)纳米粒子能够快速催化溶解氧产生活性氧自由基,从而催化无色底物TMB发生显色反应,再经过808 nm激光照射后,会产生明显的温度变化。通过进一步研究发现,再加入酚类物质后,颜色变化明显减弱,并伴随着温度变化降低,可以实现对酚类物质的特异性检测。在最优条件下,温度变化与酚类物质的浓度在0~100μmol/L范围内有着良好的线性关系,检测限为2.67μmol/L。此外该方法有着良好的抗干扰能力,不受其他结构类似有机物的影响。该方法具有操作方便、仪器设备简单易得、灵敏度好、抗干扰能力强等优点,在酚类物质的检测方面有着相当不错的应用前景。

Fe_(3)O_(4)@MnO_(2)nanoparticles with strong oxidase-like activity and high efficiency are synthesized.These nanoparticles have a number of benefits,including stability and easily manufacturing.Combined with the enzyme inhibition of phenolics,a straightforward,quick,and obvious temperature detection approach is constructed for phenolics,making up for the drawbacks of the conventional detection methods.Fe_(3)O_(4)@MnO_(2)nanoparticles may quickly catalyze the dissolved oxygen to produce reactive oxygen free radicals,therefore catalyzes the chromogenic reaction of TMB,a colorless substrate.This reaction will result in noticeable temperature increase when it is exposed to an 808 nm laser.It is shown through further study that the addition of phenolics leads to a significant weakening in color change,as well as a decrease in temperature change,allowing for the precise identification of phenolics.Under the optimal conditions,there is a linear relationship between temperature change and phenolic concentration within the range of 0-100μmol·L^(-1),along with a detection limit of 2.67μmol·L^(-1).This approach is resistant to other organic compounds with comparable structure.It has the advantages such as good sensitivity,strong anti-interference ability,easily operating,simple instrumentation,and so on,and it has a promising prospect in phenolics detection.