基于网络药理学及混合加权法优化凤仙透骨草提取工艺

Optimization of extraction process of Impatientis Balsaminae Caulis based on network pharmacology and mixture weighting methods

目的:通过网络药理学、混合加权法及响应面法优化凤仙透骨草治疗骨关节炎(OA)的提取工艺。方法:利用网络药理学及分子对接技术,对凤仙透骨草治疗骨关节炎的潜在活性成分及靶点进行筛选及验证,LC-MS确定关键成分并以各指标成分含量为评价指标,利用层次分析法(AHP)-指标相关性的指标权重确定方法(CRITIC)混合加权法及Box-Behnken响应面设计优化提取工艺。结果:东莨菪内酯、槲皮素、山柰酚为凤仙透骨草治疗骨关节炎的活性成分,三者混合提取的最佳工艺为加入5倍量82%乙醇,回流提取2次,每次35 min。结论:凤仙透骨草通过多成分、多靶点、多通路发挥治疗骨关节炎的作用,AHP-CRITIC混合加权法确定的权重系数客观合理,优选出的提取工艺稳定可行。

Objective:To optimize the extraction process of Impatientis Balsaminae Caulis in the treatment of degenerative osteoarthritis based on network pharmacology,mixture weighting and response surface method.Methods:Network pharmacology and LC-MS techniques were used to determine the active ingredients of Impatientis Balsaminae Caulis in the treatment of osteoarthritis,and they were taken as evaluation indexes.The extraction process of Impatientis Balsaminae Caulis was optimized by analytic hierarchy process(AHP)-criteria importance through intercriteria correlation(CRITIC)mixed weighting method and Box-Behnken response surface design.Results:Scopoletin,quercetin and kaempferol were the active ingredients in the treatment of osteoarthritis.The optimal extraction process was to add 5 times of 82%ethanol and reflux twice,35 min each time.Conclusion:The treatment of osteoarthritis can be achieved by regulating target genes and related signaling pathways through various active components.The weight coefficient determined by AHP-CRITIC hybrid weighting method is objective and reasonable,and the optimized extraction process is stable and feasible.