面前静脉作为移植物的大鼠颈总动脉动静脉端端吻合模型建立

Establishment of rat common carotid artery arteriovenous end-to-end anastomosis model with anterior facial vein as graft

目的建立面前静脉作为自体移植物的大鼠颈总动脉(CCA)动静脉端端吻合训练模型。方法选取24只SD大鼠并随机分为两组,分别进行经典CCA端端吻合(n=12)及面前静脉作为自体移植物的CCA动静脉端端吻合(n=12)。所有吻合均以间断吻合方式进行,建立CCA动静脉端端吻合模型。结果大鼠CCA直径为1.0~1.2 mm,面前静脉直径为0.8~1.0 mm。经典CCA端端吻合约需8~10针,平均吻合时间为(22.75±1.66)min。将面前静脉作为自体移植的CCA动静脉端端吻合组中,每只大鼠需进行2个端端吻合,总吻合时间为(49.42±2.47)min,每个吻合口需8~10针,耗时(24.71±1.65)min。所有吻合口即时通畅率及30 min后通畅率均为100%。结论面前静脉作为自体移植物进行大鼠CCA动静脉微血管端端吻合练习,可模拟血管直径及血管壁厚度均不匹配的端端吻合,该模型为经典CCA端端吻合训练模型的补充。

Objective To establish a training model of common carotid artery(CCA)anastomosis end-to-end in rats with anterior facial vein as autologous graft.Methods Twenty-four SD rats were selected and randomly divided into two groups for classical end-to-end anastomosis of CCA(n=12)and end-to-end anastomosis of CCA with anterior facial vein as autologous graft(n=12).All anastomosis were performed in a discontinuous manner to establish a CCA arteriovenous end-to-end anastomosis model.Results The diameter of CCA in rats was 1.0-1.2 mm,and the diameter of anterior facial vein was 0.8-1.0 mm.The classic CCA end-to-end anastomosis required about 8-10 needles,and the average anastomosis time was(22.75±1.66)min.In the CCA arteriovenous end-to-end anastomosis group with anterior facial vein as autologous graft,each rat needed 2 end-to-end anastomosis,the total anastomosis time was(49.42±2.47)min,each anastomosis required 8-10 needles and took(24.71±1.65)min.The immediate patency rate of all anastomosis and the patency rate after 30 min were 100%.Conclusion The anterior facial vein is used as an autologous graft for rat CCA arteriovenous microvascular end-to-end anastomosis practice,which can simulate end-to-end anastomosis with mismatched vessel diameter and wall thickness.This model is a supplement to the classic CCA end-to-end anastomosis training model.