LINC00520通过miR-519b-3p/HIF1A轴促进胃癌的侵袭转移

LINC00520 promoting the invasion and metastasis of gastric cancer through miR-519b-3p/HIF1A axis

目的探讨LINC00520在胃癌中的功能和分子机制,揭示其在胃癌发展中潜在作用。方法筛选GEO数据库中胃癌样本,寻找与胃癌恶性进展相关的差异表达基因。qPCR检测LINC00520在胃癌中的表达水平,细胞功能实验探索LINC00520对胃癌进展的潜在影响。生物信息学分析LINC00520与miR-519b-3p和HIF1A之间的关联,并利用双荧光素酶报告基因和RNA免疫沉淀实验验证它们之间的结合活性。结果胃癌中LINC00520表达显著上调(P<0.01),LINC00520过表达促进胃癌细胞的恶性进展(均P<0.01)。LINC00520通过竞争性靶向miR-519b-3p,上调HIF1A表达(均P<0.01)。结论LINC00520在胃癌中高表达,通过靶向miR-519b-3p/HIF1A轴发挥着关键调节作用,为胃癌诊断和治疗提供新的靶点。

Objective To investigate the function and molecular mechanism of LINC00520 in gastric cancer and to reveal the potential role of LINC00520 in gastric cancer development.Methods GEO database were screened for differentially expressed genes associated with malignant progression of gastric cancer.qPCR was performed to detect the expression of LINC00520 in gastric cancer,and cellular function experiments were performed to explore the potential effects of LINC00520.The association between LINC00520 and miR-519b-3p and HIF1A was detected by bioinformatics analysis,and their binding activities were verified by using dual luciferase reporter gene and RNA immunoprecipitation assays.Results In gastric cancer,the expression of LINC00520 was significantly increased(P<0.01),and LINC00520 promoted the malignant progression of gastric cancer cells(P<0.01).By bioinformatics,dual luciferase reporter gene and RNA immunoprecipitation assays,it was found that LINC00520 upregulated HIF1A expression by competitively targeting miR-519b-3p(P<0.01).Conclusion High expression of LINC00520 in gastric cancer plays a critical role by targeting the miR-519b-3p/HIF1A axis,which provides new targets for the diagnosis and treatment of gastric cancer.