花椒天然成分花椒素(WGX-50)的保湿性能及对人体皮肤屏障功能的影响

Effects of Natural Component of Zanthoxylum Xanthoxylum(WGX-50)on Moisture Retention and Skin Barrier Function

目的:探究花椒天然成分WGX-50配制剂的保湿性能及对皮肤屏障功能的影响。方法:以角质形成细胞HaCaT和16例皮肤状态良好的受试者为研究对象。HaCaT细胞用于测试WGX-50的细胞毒性,分析WGX-50对水通道蛋白3(Aquaporin 3,AQP3)、聚角蛋白微丝蛋白(Filaggrin)、闭合蛋白(Claudin-1)、透明质酸合成酶2(Hyaluronic acid synthase 2,HAS2)和CD44表达水平的影响;测试WGX-50对人体皮肤屏障功能的影响。结果:0.5μmol/L花椒素WGX-50促进HaCaT细胞增殖(P<0.05),1.0~100.0μmol/L WGX-50抑制HaCaT细胞活性,细胞活性均低于对照组(P<0.05),尤其高浓度(≥10μmol/L)WGX-50对HaCaT细胞活性抑制作用更显著(P<0.01)。0.5μmol/L的WGX-50能有效促进AQP3基因表达(P<0.05),但对Filaggrin、Claudin-1、HAS2、CD44基因表达水平无明显影响(P>0.05)。皮肤水分含量和经表皮水分散失速率实验显示,花椒素WGX-50对皮肤具有良好的保湿效果。结论:低浓度花椒素(0.5μmol/L)WGX-50可促进HaCaT细胞增殖,增强HaCaT细胞内AQP3基因表达,改善人体皮肤屏障功能。

Objective To study the moisturizing properties of the natural component of Zanthoxylum WGX-50 and its effect on skin barrier function.Methods Keratinocyte HaCaT and 16 subjects with good skin condition were studied.HaCaT cells were used to test the cytotoxicity of WGX-50,and the effects of WGX-50 on the expression levels of aquaporin 3(AQP3),Filaggrin,Claudin-1,hyaluronic acid synthetase 2(HAS2)and CD44 were analyzed.Subjects were used to test the effects of WGX-50 on skin barrier function.Results 0.5μmol/L WGX-50 promoted HaCaT cell proliferation(P<0.05),and 1.0-100.0μmol/L WGX-50 inhibited HaCaT cell activity,and the cell activity was lower than that of control group(P<0.05).In particular,high concentration of WGX-50(≥10μmol/L)significantly inhibited the activity of HaCaT cells(P<0.01).0.5μmol/L WGX-50 could effectively promote AQP3 gene expression(P<0.05),but had no significant effect on Filaggrin,Claudin-1,HAS2 and CD44 gene expression levels(P>0.05).Experiments on skin moisture content and transepidermal moisture loss rate showed that xanthoxylin WGX-50 had good moisturizing effect on skin.Conclusion Low concentration of xanthoxylin(0.5μmol/L)WGX-50 can promote the proliferation of HaCaT cells,enhance the expression of AQP3 gene in HaCaT cells,and improve the function of human skin barrier.