GmMYB15正调控大豆对大豆孢囊线虫的抗性

GmMYB15 positively regulates soybean immunity to cyst nematode

孢囊线虫为大豆根部重要病害之一,广泛分布于我国大豆种植区,制约我国大豆产业发展。种植抗病品种为该病害最为经济有效且绿色环保的防治策略,然而目前商业化抗性资源较少。解析大豆抗孢囊线虫的机制有利于大豆抗性基因资源挖掘和抗线虫分子育种。基于生化试验和抗线虫表型分析,本研究发现大豆R2R3-MYB转录因子GmMYB15在植物细胞质和细胞核中表达,其转录水平受大豆孢囊线虫侵染显著诱导,GmMYB15与咖啡酰辅酶A氧甲基转移酶CCoAOMT、肉桂酸羟化酶C4H和肉桂酰辅酶A还原酶CCR等蛋白互作,正调控大豆对大豆孢囊线虫抗性。研究结果为后续深入研究GmMYB15调控大豆线虫抗性机制奠定了基础。

The soybean cyst nematode,a major threat to soybean roots,is widely distributed in soybean growing areas and restricts the development of the soybean industry in China.Planting disease-resistant varieties is the most economically effective and environment-friendly control strategy for this disease.However,there are currently few commercially available resistance resources.Understanding the molecular mechanism of soybean resistant to cyst nematode is essential for exploring soybean resistance gene resources and advancing molecular breeding for nematode resistance.Based on biochemical experiments and phenotypic analysis,we found that the R2R3-MYB transcription factor GmMYB15 is expressed in both the cytoplasm and nucleus,with its increased transcription level under cyst nematode infection.GmMYB15 interacts with various proteins,such as caffeoyl coenzyme A O-methyltransferase(CCoAOMT),cinnamate-4-hydroxylase(C4H),and cinnamoyl coenzyme A reductase(CCR),and plays a positive role in soybean nematode resistance.Our findings lay a foundation for in-depth researches on the function of GmMYB15 in regulating soybean nematode resistance.