腐烂茎线虫经羊消化道后的活性检测与分子鉴定

Detection and molecular identification of Ditylenchus destructor in goat feces

腐烂茎线虫是严重威胁我国马铃薯、甘薯等产业发展的重要植物病原线虫,而目前对其传播机制仍不清楚。本研究收集田间感染马铃薯腐烂茎线虫的病薯饲喂山羊,结果在羊粪中检测到了腐烂茎线虫虫卵。分子鉴定结果表明,相关病原线虫为B型腐烂茎线虫。通过孵化试验与室内接种试验进一步证明了羊粪中的腐烂茎线虫虫卵可以孵化成幼虫为害马铃薯。相关研究首次证实了粪肥对腐烂茎线虫的传播作用,揭示了榆林地区常年连作玉米的田块在种植马铃薯后腐烂茎线虫病突然暴发的潜在原因,对于今后指导种植户及时清理、集中销毁田间病烂薯,以及杜绝使用未腐熟羊粪作为肥料的粗放管理模式提供了重要理论依据。

Ditylenchus destructor,a significant plant parasitic nematode,poses a severe threat to potato and sweet potato industries in China,yet its transmission mechanism remains elusive.In this study,diseased potatoes infected with D.destructor collected from fields were used to feed goats.Consequently,D.destructor eggs were detected in the goats’feces.Molecular identification confirmed that the pathogenic nematodes from the diseased potatoes belonged to haplotype B of D.destructor.Incubation and inoculation tests demonstrated that D.destructor eggs present in goat manure were capable of hatching into larvae and infecting potatoes.This study revealed for the first time that D.destructor can be transmitted through goat feces,uncovering a potential cause for sudden outbreaks of D.destructor disease in potato fields following perennial continuous corn crops in the Yulin area.These findings provide crucial theoretical support for future recommendations,including the prompt removal of diseased and rotten potatoes from fields,centralized disposal,and the strict avoidance of using un-composted goat feces as fertilizer.