不同生长期滨海白首乌叶主要成分与生物活性分析

Analysis and application of the main functional components and biological activity of the Cynanchum auriculatum Royle ex Wight leaf in different growth stages

[目的]明确滨海白首乌叶的功能成分和活性作用,资源化利用滨海白首乌叶。[方法]以不同生长时期滨海白首乌叶为研究材料,测定滨海白首乌叶发芽期(五月)、生长期(八月)、落叶期(十月)3个不同时期的主要功能成分的变化规律,分析比较其抗氧化能力和降血糖活性,并利用首乌叶制成一款具有保健功效的叶茶。[结果]滨海白首乌五月、八月、十月叶中多糖含量分别为13.803,3.064,3.809 mg/g,C_(21)甾体苷含量分别为8.973,19.640,2.653 mg/g,均显著低于首乌块根;其黄酮含量分别为13.407,17.776,2.022 mg/g,显著高于首乌块根。白首乌八月叶提取液对α-葡萄糖苷酶的抑制率和抗氧化能力均高于滨海白首乌块根。滨海白首乌叶茶中活性物质含量较高,具有抗氧化和降血糖活性。[结论]滨海白首乌叶中含有较高的功能成分和生物活性物质,是一种能够高值化利用的生物资源。

[Objective]This study aimed to clarify the functional components and active effects of Cynanchum auriculatum Royle ex Wight leaves,and make use of C.auriculatum Royle ex Wight leaves as resources.[Methods]Taking the leaves of C.auriculatum Royle ex Wight at different growth stages as the research material,the changes of the main functional components in the leaves of C.auriculatum Royle ex Wight were measured in three different stages:germination stage(May),growth stage(August)and deciduous stage(October).The antioxidant capacity and hypoglycemic activity of the leaves were analyzed and compared,and the leaves were used to make a leaf tea with health care functions.[Results]The polysaccharide content and C_(21)steroid glycoside content in the leaves of C.auriculatum Royle ex Wight in May,August and October were 13.803,3.064 and 3.809 mg/g,respectively,which were significantly lower than those in the roots of C.auriculatum Royle ex Wight.The flavonoid contents were 13.407,17.776 and 2.022 mg/g,respectively,which were significantly higher than those of C.auriculatum Royle ex Wightroot.The inhibition rate ofα-glucosidase and antioxidant capacity of August leaf extract were higher than those oftuber.C.auriculatum Royle ex Wight leaf tea has high content of active substances,which has antioxidant and hypoglycemic activities.[Conclusion]The C.auriculatum Royle ex Wight leaves have high functional components and bioactive substances,making them highly valuable for use.