白酒糟中ACE抑制肽提取工艺优化及抗氧化活性

Optimization of the extraction process of ACE inhibitory peptides from distillery waste and their antioxidant activity

[目的]有效处理白酒糟,研究和开发白酒糟中的高附加值成分,探索其潜在应用途径。[方法]采用超声波辅助碱提酸沉法从白酒糟中提取蛋白质,利用复合酶对白酒糟粗蛋白进行酶解制备血管紧张素转换酶(ACE)抑制肽,通过单因素试验和响应面试验优化ACE抑制肽的提取工艺条件,并对其抗氧化活性进行研究。[结果]采用复合酶法制备ACE抑制肽得到了最佳条件:酶配比(V_(木瓜蛋白酶)∶V_(中性蛋白酶))3∶1,水解温度55.41℃,水解pH 7.72,水解时间2.38 h,该条件下ACE抑制肽水解度为66.62%;复合蛋白酶酶解3 h后得到最高的ACE抑制活性(79.05%);经提取纯化后的ACE抑制肽对DPPH自由基和ABTS自由基的清除率分别为2.078%和3.226%,总抗氧化能力分别为1.161μmol/mL和1.255 mmol。[结论]采用复合酶法从白酒糟中提取ACE抑制肽工艺可行。

[Objective]This study aimed to handle distillery waste effectively,research and develop high-value components within distillery waste,and explore its potential application approaches.[Methods]Proteins were extracted from distillery waste throuth the ultrasonicassisted alkali extraction and acid precipitation method.Angiotensin-converting enzyme(ACE)inhibitory peptides were prepared by enzymatic hydrolysis of the crude protein of distillery waste using a compound enzyme.The extraction process conditions of ACE inhibitory peptides were optimized by single factor experiments and response surface experiments,and their antioxidant activity was investigated.[Results]The optimal conditions for preparing ACE-inhibitory peptided by compound enzymatic hydrolysis were as follows:the ratio of enzyme(V_(papainase)∶V_(neutral protease))was 3∶1,and the hydrolysis was contacted at 55.41℃and pH 7.72,for 2.38 h.Under the control of these conditions,the hydrolysis degree of ACE-inhibitory peptide was 66.62%;the highest ACE inhibitory activity(79.05%)was obtained after 3 h of enzymatic hydrolysis.The scavenging rates of the purified and extracted ACE inhibitory peptides against DPPH and ABTS free radicals were 2.078%and 3.226%,respectively,and the total antioxidant capacities were 1.161μmol/mL and 1.255 mmol.[Conclusion]The process of extracting ACE inhibitory peptide from distillery waste using the compound enzymatic hydrolysis method is feasible.