鸡胆汁酶转化物对大鼠急性酒精性肝损伤的保护作用

Protective Effect of Enzymatically Processed Chicken bile on Acute Alcoholic Liver Damage in Rats

该实验旨在探究鸡胆汁酶转化物(CB)对大鼠酒精性肝病(ALD)的保护作用,并揭示其作用机制。35只8周龄的雄性大鼠适应性喂养一周后,随机分为正常组、模型组、CB低中高剂量(50、100、150 mg/kg)。经过一周6 mL/kg·bw的酒精造模及CB干预后处死大鼠收集血清及肝脏测定相关指标,通过H&E染色观察肝组织形态。结果表明,高剂量CB具有良好的护肝效果,改善了大鼠体质量的降低及肝脏指数的升高。高剂量CB干预后,肝脏中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)和过氧化氢酶(CAT)活性分别提高67.48%、110.07%、34.30%,细胞色素酶(CYP2E1)水平降低35.02%,酒精代谢能力显著提高,同时,血清中谷草转氨酶(AST)、碱性磷酸酶(ALP)和谷丙转氨酶(ALT)活性分别降低35.62%、38.50%、45.25%,改善肝脏损伤。另外,高剂量CB干预可降低血清中51.23%总胆固醇(TC)和47.12%甘油三酯(TG)的水平,提高脂质代谢,同时,肝脏中超氧化物歧化酶(SOD)活力提高102.93%,谷胱甘肽(GSH)水平提高40.69%,丙二醛(MDA)水平降低35.85%,减缓肝脏氧化应激。高剂量CB干预可下调TLR4/MyD88/NF-κB/IKBα信号通路的基因和蛋白的表达,且白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)水平分别降低31.29%、28.71%、40.48%和37.48%,减缓炎症应激。研究结果表明,CB具有开发成为护肝功能性食品及保健品的潜力。

The protective effects of enzymatically converted chicken bile(CB)on alcoholic liver diseases(ALD)in rats and its potential mechanism were investigated.Thirty-five 8-week-old male rats were randomly divided into a control group;a model group;and low-,medium-and high-dose CB treatment groups(50,100,and 150 mg/kg,respectively)after adaptive feeding for 1 week.At 1 week after modeling using 6 mL/kg·bw of alcohol and CB intervention,all rats were sacrificed.Serum and liver samples were collected to determine relevant indices,and the liver histology was observed using hematoxylin and eosin staining.The results showed that a high dose of CB was associated with hepatoprotective effects by ameliorating the reduction in body weight and elevated liver index in rats. After high-dose CB intervention, the activities of hepatic alcohol dehydrogenase, acetaldehyde dehydrogenase, and catalase were increased by 67.48%, 110.07%, and 34.30%, respectively, whereas the cytochrome enzyme level decreased by 35.02%, indicating a substantial enhancement in alcohol metabolism. Moreover, the activities of alanine transaminase, alkaline phosphatase, and alanine transaminase in the serum were reduced by 35.62%, 38.50%, and 45.25% respectively, indicating improvements in liver damage. In addition, CB intervention reduces the levels of total cholesterol (by 51.23%) and triglycerides (by 47.12%) in the serum, increased superoxide dismutase activity (by 102.93%) and the glutathione level (by 40.69%), and decreased the malondialdehyde level (by 35.85%) in the liver. These results suggest that lipid metabolism was enhanced and oxidative stress was alleviated. High- dose CB intervention downregulates the expression levels of genes and proteins involved in the TLR4/MyD88/NF-κB/IKBα signaling pathway. The expression levels of interleukin 1β, interleukin 6, interleukin 8, and tumor necrosis factor α were reduced by 31.29%, 28.71%, 40.48%, and 37.48%, respectively, indicating reduced inflammatory stress. Based on these findings, CB shows potential for development as a functional food and health care product for liver protection.