肠易激综合征患者血清miR-219a-5p和miR-338-3p水平与肠道微生物及炎症因子水平的关系

Relationship of serum miR-219a-5p and miR-338-3p level with intestinal microbiota and inflammatory factors level in patients with irritable bowel syndromes

目的探讨肠易激综合征(IBS)患者血清微小RNA-219a-5p(miR-219a-5p)和微小RNA-338-3p(miR-338-3p)水平与肠道微生物及炎症因子水平的关系。方法选取2019年10月至2022年10月湖北民族大学附属民大医院收治的90例IBS患者作为观察组,另选取同期在该院体检的90例健康者作为对照组。比较两组血清miR-219a-5p和miR-338-3p水平、肠道微生物数量及炎症因子[高敏C-反应蛋白(hs-CRP)、白细胞计数(WBC)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-1α、IL-1β、IL-10]水平。采用Pearson相关分析IBS患者血清miR-219a-5p、miR-338-3p水平与肠道微生物数量、炎症因子水平及Chao 1指数的相关性。结果观察组血清miR-219a-5p、miR-338-3p水平及Chao I指数明显低于对照组(P<0.05)。观察组双歧杆菌、乳酸杆菌数量低于对照组,大肠埃希菌、链球菌数量高于对照组,差异均有统计学意义(P<0.05)。观察组hs-CRP、TNF-α、IL-6、IL-1α、IL-1β水平及WBC高于对照组,IL-10水平低于对照组,差异均有统计学意义(P<0.05)。Pearson相关性分析结果显示,IBS患者血清miR-219a-5p、miR-338-3p水平与双歧杆菌、乳酸杆菌数量及IL-10水平均呈正相关(P<0.05),与大肠埃希菌、链球菌数量及hs-CRP、TNF-α、IL-6、IL-1α、IL-1β、WBC及Chao 1指数均呈负相关(P<0.05)。结论IBS患者血清miR-219a-5p、miR-338-3p水平与肠道微生物数量及炎症因子水平有相关性,可能成为IBS诊断和治疗的血清指标。

Objective To investigate the relationship of serum microRNA-219a-5p(miR-219a-5p)and microRNA-338-3p(miR-338-3p)level with intestinal microbiota and inflammatory factors level in patients with irritable bowel syndrome(IBS)and gut microbiota and inflammatory factors.Methods From October 2019 to October 2022,90 IBS patients diagnosed and treated in the Affiliated Minda Hospital of Hubei University for Nationalities were regarded as the observation group,90 healthy individuals who underwent health checks in the hospital were regarded as the control group.The levels of serum miR-219a-5p and miR-338-3p,the number of intestinal microbiota and inflammatory factors,including high-sensitivity C-reactive protein(hs-CRP),white blood cell count(WBC),tumor necrosis factor-α(TNF-α),interleukin(IL)-6,IL-1α,IL-1βand IL-10 in the two groups were compared.Pearson′s correlation was used to analyze the correlation of serum miR-219a-5p and miR-338-3p levels with the number of intestinal microbiota,the levels of inflammatory factors and Chao 1 index.Results The serum levels of miR-219a-5p and miR-338-3p and Chao 1 index in the observation group were obviously lower than those in the control group(P<0.05).The levels of Bifidobacterium and Lactobacillus in the feces of IBS patients in the observation group were lower than those in the control group,while the levels of Escherichia coli and Streptococcus were higher than those in the control group,with statistically significant differences(P<0.05).The levels of hs-CRP,TNF-α,IL-6,IL-1α,IL-1βand WBC in the observation group were higher than those in the control group,while the level of IL-10 was lower than that in the control group,with statistically significant differences(P<0.05).The results of Pearson′s correlation analysis showed that serum miR-219a-5p,miR-338-3p levels in IBS patients were both positively correlated with the number of bifidobacteria,Lactobacillus and IL-10 level(P<0.05),and negatively correlated with the number of E.coli,Streptococcus,and hs-CRP,TNF-α,IL-6,IL-1α,IL-1βand WBC levels(P<0.05).Spearman′s correlation analysis results showed that serum miR-219a-5p and miR-338-3p levels were negatively correlated with Chao 1 index(P<0.05).Conclusion Serum miR-219a-5p and miR-338-3p levels in IBS patients correlate with the number of intestinal microbiota and the level of inflammatory factors levels,which may become serum indicators for the diagnosis and treatment of IBSS.