多通道小鼠无创血压监测及影响因素探讨

Multi channel non-invasive blood pressure monitoring in mice and exploration of the influencing factors

目的:探索一套多通道、高灵敏、智能化的小鼠无创血压监测方案,实现小鼠血压群体监测及数据自动收集与分析。方法:将30只18~20 g雄性C57BL/6小鼠随机分为高血压组Ⅰ、高血压组Ⅱ和空白对照组,每组10只。在小鼠高血压成模过程中,使用CODA血压监测设备连续8周监测小鼠外周血压,收集和分析监测数据,探讨可能存在的影响因素,改进和优化血压监测方案。结果:按照本方案,我们实现每次同时对8只小鼠进行血压监测,单次监测时间为4~5 min,操作过程简便,实用性强,小鼠应激小且状态平稳。高血压组Ⅰ、高血压组Ⅱ及空白对照组小鼠血压监测数据均符合正态分布,且标准差(σ)小于平均值的10%。数据整体较为集中,离散程度较低,表明CODA血压监测设备具备良好的稳定性和准确性。高血压组Ⅰ及高血压组Ⅱ小鼠血压与对照组相比均明显升高,且在第7~8周时达到峰值。结论:本研究提出的小鼠无创血压监测方案具有易于操作、无创无痛、监测效率高、数据准确等诸多优势,能够实现小鼠血压快速批量监测,解决当前生物医药研究领域中小鼠血压批量监测的难题,适于大范围推广应用。与此同时,本方案从多个方面减小血压监测中相关因素影响,最大程度减小实验误差,保障实验动物福利伦理。

Objective:To explore a multi-channel,highly sensitive,and intelligent non-invasive monitoring plan for mice blood pressure,achieving group monitoring of mouse blood pressure and automatic data collection and analysis.Methods:A total of 30 male C57BL/6 mice weighing 18-20 g were randomly divided into hypertension groupⅠ,hypertension groupⅡ,and blank control group,with 10 mice in each group.During the modeling process of hypertension in mice,CODA blood pressure monitoring equipment was used to continuously monitor the peripheral blood pressure of mice for 8 weeks;The monitoring data were collected and analyzed to explore possible influencing factors,and to improve and optimize the blood pressure monitoring plan.Results:According to this plan,we achieved simultaneous blood pressure monitoring of 8 mice each time,with a single monitoring time of 4-5 min.The operation process is simple and practical,and the mice have low stress and stable state.The blood pressure monitoring data of hypertensive group Ⅰ , hypertensive group Ⅱ , and blank control groupmice all followed a normal distribution, and the standard deviation(σ) was less than 10% of the mean.The overall data was relatively concentrated with low dispersion, indicating that the CODAmonitoring equipment has good stability and accuracy. The blood pressure of mice in hypertensivegroup Ⅰ and hypertensive group Ⅱ was significantly increased as compared with that in the controlgroup, and reached the peak after 7 or 8 weeks. Conclusion: The non - invasive monitoring plan formice blood pressure proposed in this study has many advantages such as easy operation, non-invasion,high monitoring efficiency, and accurate data. It can achieve rapid batch monitoring of mouse bloodpressure, solve the problem of batch monitoring of mice blood pressures in the current field of biomedicineresearch, and is suitable for large-scale promotion and application. At the same time, this plan reducesthe influence of relevant factors in blood pressure monitoring from multiple aspects, minimizesexperimental errors to the greatest extent, and ensures the welfare and ethics of experimental animals.