炙红芪-醋莪术对结肠炎相关结肠癌小鼠短链脂肪酸代谢的影响

Effects of Hedysari Radix Praeparata Cum Melle and vinegar processed Curcumae Rhizomaon short-chain fatty acid metabolism in colitis-related colon cancer mice

目的探究炙红芪-醋莪术对结肠炎相关结肠癌(CAC)小鼠短链脂肪酸(SCFAs)的影响。方法将40只C57BL/6小鼠随机分为空白组、模型组、柳氮磺胺吡啶组和炙红芪-醋莪术组,每组10只。空白组灌胃给予等量饮用水,其余组用氧化偶氮甲烷联合葡聚糖硫酸钠建立CAC模型。柳氮磺胺吡啶组和炙红芪-醋莪术组从实验开始就灌胃给予0.455g·kg^(-1)柳氮磺胺吡啶和6.825g·kg^(-1)炙红芪-醋莪术溶液进行干预。用气相色谱-质谱法检测小鼠结肠内容物中SCFAs的含量。结果空白组小鼠结肠内容物中乙酸、丙酸、异丁酸、丁酸、戊酸和己酸的含量分别为(2.80±0.54)、(0.47±0.26)、(0.02±5.90×10^(-3))、(0.82±0.24)、(9.85±2.24)×10^(-3)和(5.45±4.26)×10^(-3)mg·g^(-1)模型组小鼠结肠内容物中乙酸、丙酸、异丁酸、丁酸、戊酸和己酸的含量均较空白组显著降低,分别为(0.71±0.36)、(0.24±0.08)、(6.08±2.54)×10^(-3)、(0.13±0.03)、(3.64±2.14)×10^(-3)和(1.99±0.81)×10^(-3)mg·g^(-1)(均P<0.01)。经炙红芪-醋莪术干预后,结肠内容物中乙酸、丙酸、丁酸、戊酸和己酸的含量均较模型组显著升高,分别为(1.58±0.25)、(0.39±0.04)、(0.34±0.08)、(5.51±1.76)×10^(-3)和(6.67±1.73)×10^(-3)mg·g^(-1)(P<0.05,P<0.01)。结论炙红芪-醋莪术可以调控CAC小鼠SCFAs的代谢。

Objective To investigate the effects of Hedysari Radix Praeparata Cum Melle and vinegar processed Curcumae Rhizoma(HRCR)on short-chain fatty acids(SCFAs)in colitis associated colon cancer(CAC)mice.Methods Forty C57BL/6 mice were randomly divided into control group,model group,sulfasazine group(SASP)and HRCR group,with 10 mice per group.Control group was given the same amount of drinking water,and the other group was given azoxymethane combined with dextran sulfate sodium to establish an CAC model.The SASP and HRCR groups were administrated with 0.455 g·kg^(-1)sulfasalazine and 6.825 g·kg^(-1)HRCR from the beginning of the experiment.The content of SCFAs in colon contents of mice was detected by gas chromatography-mass spectrometry.ResultsTThe contents of acetic acid,propionic acid,isobutyric acid,butyric acid,valeric acid,and hexanoic acid in the colon contents of control group mice were(2.80±0.54),(0.47±0.26),(0.02±5.90×10^(-3)),(0.82±0.24),(9.85±2.24)×10^(-3),(7.21±4.21)×10^(-3)mg·g^(-1)respectively.Compared with the control group,the contents of acetic acid,propionic acid,isobutyric acid,butyric acid and valeric acid in the colon contents of mice in the model group were significantly reduced[(0.71±0.36),(0.24±0.08),(6.08±2.54)×10^(-3),(0.13±0.03),(3.64±2.14)×10^(-3)and(1.99±0.81)×10^(-3)mg·g^(-1),all P<0.01].Compared with the model group,the contents of acetic acid,propionic acid,butyric acid,valeric acid,and hexanoic acid in the colon contents of mice treated with HRCR were significantly increased after intervention[(1.58±0.25),(0.39±0.04),(0.34±0.08),(5.51±1.76)×10^(-3)and(6.67±1.73)×10^(-3)mg·g^(-1),P<0.05,P<0.01].Conclusion The metabolism of SCFAs in CAC mice can be regulated by decocted astragalus-vinegar Curcuma.