MYB通过调控细胞周期促进胃癌细胞的增殖

MYB promotes the proliferation of gastric cancer cells by regulating cell cycle

目的探讨MYB基因在胃癌细胞增殖过程中的作用及机制。方法应用免疫组化实验在200对胃癌配对组织中检测MYB的表达;利用MTT和克隆形成实验检测MYB对胃癌细胞增殖的影响;采用流式细胞术研究MYB对细胞周期的影响;应用TCGA转录组数据分析MYB对细胞周期相关基因表达的影响。结果MYB在胃癌组织表达显著增加,且与患者预后负相关;MYB通过调控细胞周期促进胃癌细胞的增殖,并且MYB的表达与周期素CYCLIN A2 mRNA的表达正相关。结论MYB可作为胃癌的诊治靶点。

Objective To explore the function and mechanism of MYB gene in the development of gastric cancer.Methods Immunohistochemistry was performed to detect the expression of MYB in 200 pairs of gastric cancer tissues.MTT and clone formation assay were used to detect the effect of MYB on the proliferation of gastric cancer cells.Flow cytometry was conducted to explore the effect of MYB on cell cycle.TCGA database was used to analyze the effect of MYB on the expression of cell cycle related genes.Results Compared with normal tissues,the expression level of MYB in gastric cancer tissues was significantly increased,and it was negatively correlated with the survive of the patients with gastric cancer.MYB promoted the proliferation of gastric cancer cells by regulating cell cycle.The expression of MYB was positively correlated with the expression of CYCLIN A2 mRNA.Conclusion MYB can be used as a biomarker for diagnosis and treatment of gastric cancer.