m1A RNA甲基化对直肠腺癌的诊断效能分析

Analysis of the diagnostic efficacy of m1A RNA methylation in rectal adenocarcinoma

目的探索m1A相关基因表达和血浆m1A总体甲基化水平对直肠腺癌诊断和预后的预测价值。方法根据Sangerbox(基于TCGA和GTEx数据库)在线平台分析11个m1A甲基化相关基因在直肠腺癌中的差异表达,结合临床数据进行诊断效能和生存分析。同时,通过检测血浆m1A甲基化水平在直肠腺癌患者和正常人中差异情况,分析其对直肠腺癌的诊断价值。结果TRMT6,一种关键的m1A编码器基因,在不同病理状态的直肠腺癌中升高最为显著,并可作为直肠腺癌,特别是早期直肠腺癌的诊断标志物,其特异性和灵敏性高达82.86%和90.00%,而且TRMT6的高表达也预示着直肠腺癌预后较好(HR=0.22,95%CI:0.08~0.60,P=0.0014)。同时,血浆m1A甲基化水平在直肠腺癌相对于正常人显著性升高,对早期直肠腺癌有一定的诊断价值,其AUC约为0.8。结论m1A相关基因和血浆m1A甲基化在直肠腺癌中升高,可作为其潜在的早期诊断标志物。本研究为直肠腺癌早期诊断提供新的靶标,将有助于指导制定更有效的治疗策略。

Objective To explore the predictive value of m1A-related gene expression and overall plasma m1A methylation levels for the diagnosis and prognosis of rectal adenocarcinoma(READ).Methods Based on the Sangerbox(an online platform based on the TCGA and GTEx databases),the differential expression of 11 m1A methylation-related genes in READ was analyzed,and diagnostic efficacy and survival analysis were conducted in combination with clinical data.Meanwhile,the difference in plasma m1A methylation levels between READ patients and healthy individuals was detected to analyze its diagnostic value.Results TRMT6,a key m1A writer gene,was most significantly elevated in READ of different pathological states,and it can serve as a diagnostic marker,especially early stage READ,with a specificity and sensitivity of up to 82.86%and 90.00%,respectively.Moreover,high expression of TRMT6 also indicated a better prognosis for READ(HR=0.22,95%CI:0.08-0.60,P=0.0014).Furthermore,plasma m1A methylation levels were significantly elevated in READ compared to healthy individuals,showing certain diagnostic value for early stage of READ,with AUC of about 0.8.Conclusion m1A-related genes and plasma m1A methylation are elevated in READ and can serve as potential early diagnostic markers.This study provided new targets for the early diagnosis of READ,which will help guide the development of more effective treatment strategies.