SCoT标记分析节瓜育种材料的遗传多样性

SCoTMarker Analysis ofGenetic Diversity in Chieh-qua BreedingMaterials

节瓜育种材料间存在着同名异种或者异名同种的问题,为了区别种质材料间的差异,对所得节瓜材料群体进行遗传多样性分析是重要的辅助手段。通过利用前期建立并优化的适合以节瓜基因组DNA为模板进行SCoT-PCR扩增的体系,利用前期工作筛选的14条SCoT分子标记对36份节瓜材料的基因组DNA进行PCR扩增。扩增后经1.5%琼脂糖凝胶电泳,UVP成像,共得到87条谱带,其中多态性的条带占78条,多态性比例为89.77%。通过聚类分析得到36份节瓜材料的遗传相似系数介于0.59~1.00之间,说明多态性位点较多,这些节瓜材料的遗传差异较大,材料间存在着异名同种的现象。当遗传相似系数为0.80时,这些节瓜材料分成6个居群,其中JG-7、JG-9、JG-32、JG-36都各自成为一个群组,这说明JG-7、JG-9、JG-32、JG-36这4个材料对整体材料的遗传背景差异起到了重要的作用。通过对这36份节瓜材料的遗传多样分析,明确了这些材料之间的遗传差异与相似性程度,为后期的育种工作亲本选择以及基因连锁图谱的构建提供了参考。

There is a problem that the different breeding materials of chieh-qua have the same name or different names belong to the same species.In order to distinguish the differences among germplasm materials,it is an important auxiliary means to analyze the genetic diversity of the chieh-qua material population collected and breeded.By using the SCoT-PCR amplification system established and optimized in the early stage,14 SCoT molecular markers were used as primers,and 36 genomic DNAs of chieh-qua materials were used as templates for PCR amplification.The amplification products of SCoT-PCR were subjected to 1.5%agarose gel electrophoresis and UVP imaging,and 87 bands were obtained,of which 78 bands were polymorphic,and the proportion of polymorphism was 89.77%.Through cluster analysis,the genetic similarity coefficient of 36 chieh-qua materials is between 0.59 and 1.00,indicating that there are many polymorphic sites,and these chieh-qua materials have great genetic differences,and there is a phenomenon of different names but belong to same species among them.When the genetic similarity coefficient is 0.80,these materials are divided into 6 populations,among which JG-7,JG-9,JG-32 and JG-36 all belong to a group respectively.This indicated that four materials,JG-7,JG-9,JG-32 and JG-36,played an important role in the genetic background difference of the whole material.By analyzing the genetic diversity of these 36 chieh-qua materials,the genetic differences and similarity among these materials were clarified,which provided reference for the selection of parents and the construction of gene linkage map in later breeding work.