番鸭羽色遗传差异基因筛选及验证研究

The screening and validation of genes differentially expressed in feather color inheritance of Muscovy duck

为探究番鸭羽色调控机制,本试验以黑羽及白羽番鸭为研究素材,运用Illumina Hiseq高通量测序技术,对不同羽色部位毛囊组织进行转录组测序,经基因本体论(GO)富集分析对差异表达基因功能进行解析,实时荧光定量逆转录PCR(RT-qPCR)验证初步获得的上调差异基因,并开展选育后代羽色性状验证筛选研究。结果:以|log_(2)FC|≥6(P<0.05)为筛选条件,获得显著差异表达基因112个(上调基因63个,下调基因49个);进一步分析获得番鸭羽色性状相关候选差异表达基因10个,胶原肌丝生成相关基因可能参与番鸭黑白羽色性状遗传的调控过程,并初步筛选肌节蛋白(MYOT)、肌红蛋白(MB)、5′核苷酸酶1型(NT5DC1)等3个上调基因;开展选育后代番鸭羽色性状表达,筛选的MYOT、MB可作为番鸭羽色遗传的标记候选基因。研究结果丰富了番鸭羽色性状遗传方面的基础性研究,对开展黑羽型番鸭育种具有积极的促进意义和研究支撑。

In order to further explore the regulation mechanism of feather color of Muscovy ducks,the experiment used black feathered and white feathered Muscovy ducks as research subjects.The transcriptome sequencing of feather follicle tissues of black and white Muscovy ducks of different feather color positions were performed by the Illumina Hiseq high throughput sequencing technique.The functions of differentially expressed genes were determined by GO enrichment analysis.RT-qPCR was used ot verify the up-regulated genes obtained initially;and the verification and screening research was conducted on feather color traits of selective breeding of Muscovy duck offspring.The results showed that the study obtained 112 significantly differentially expressed genes(63 up-regulated genes,and 49 down-regulated genes),with the ab⁃solute value of|log_(2)FC|≥6(P<0.05)as the screening condition.The further analysis obtained 10 candidate differentially expressed genes related to the feather color trait of Muscovy ducks.The genes related to collagen myofilament production might be involved in the genet⁃ic regulation of black or white plumage traits of Muscovy ducks;and three up-regulated genes,MYOT,MB,NT5DC1,were initially screened here.In the expression of feather color traits of Muscovy ducks,MYOT and MB were screened as marker candidate genes for Musco⁃vy ducks feather color inheritance.The present research results enriched the basic research on the inheritance of plumage traits of Muscovy ducks,were of positive promotion significance,and would serve as support for research on the breeding of black feathered Muscovy ducks.