自拟催情微粉的质控及药效学研究

Study on the quality standards and pharmacodynamics of self-formulated aphrodisiac micropowder

旨在制定自拟催情微粉的质量标准并验证其促进生殖系统发育的药效学。采用薄层色谱法对样品中的当归、α-香附酮进行鉴别;采用高效液相色谱法对淫羊藿苷的含量进行测定;采用小鼠子宫重量法测定自拟催情微粉的药效学。结果:自拟催情微粉供试品的色谱中,样品与当归、α-香附酮对照品相应的位置上均显示相同颜色的斑点,阴性对照品则无,专属性强,建立了当归、α-香附酮的鉴别方法;淫羊藿苷含量测定显示呈线性关系良好(R2=0.997 5),平均加样回收率为98.87%,10批中试产品的淫羊藿苷平均含量为0.15%,将自拟催情微粉含量限度定为淫羊藿苷不得少于0.12%;与空白对照组比较,每天给药自拟催情微粉24、8 g/kg剂量组可以显著提高小鼠子宫器官系数。综上,建立的检测方法可行、稳定,可用于自拟催情微粉的质量控制,自拟催情微粉具有促进小鼠生殖系统发育的作用。

This study was to establish a quality standard of self-formulated aphrodisiac powder and verify its pharmacological effects in pro⁃moting reproductive system development.The thin layer chromatography method was used to determine the content of angelica sinensis andα-cyperone.The content of icariin was determined by High-performance liquid chromatography.And pharmacodynamics of self-formulated aph⁃rodisiac powder was determined using the mouse uterine weight method.The results showed that,in chromatography,the test samples had the same color with angelica sinensis andα-cyperone,while the negative control samples did not.The content determination results showed that icariin had good linearity(R2=0.9975),at an average recovery rate of 98.87%.The content of icariin in 10 batches was at an average of 0.15%.The content limit of icariin was no less than 0.12%.Compared with the blank control group,daily administration of micropowder at doses of 24 g/kg and 8 g/kg could significantly improve the uterine organ coefficient of mice.The results indicated that the detection method was accurate and reliable,and it could be used for the quality evaluation of self-formulated aphrodisiac powder.It had the effect of promoting the development of the reproductive system in mice.The established detection method was feasible and stable,and self-formulated aphrodisi⁃ac powder had the effect of promoting the development of the mouse reproductive system.