猪流感二价灭活疫苗对H1N1亚型流行毒株的攻毒保护效果

Swine influenza bivalent inactivated vaccine for the cross protection effect of a pandemic H1N1 subtype strain in 2021

旨在评价猪流感二价灭活疫苗(H1N1 LN株+H3N2 HLJ株)对2021年国内H1N1亚型猪流感流行毒株的交叉保护效果。将10头4周龄健康易感猪随机均分为疫苗接种组和对照组,疫苗接种组每头接种疫苗2 mL,首免14 d后进行二免,对照组未接种疫苗;首免后28 d,每头猪经气管内注射A/Swine/Jiangsu/NL33/2021(H1N1)(简称NL33株)4.0 mL;综合临床症状、鼻拭子病毒分离和肺脏病变3个方面进行判定。结果:试验组保护率为80%,对照组发病率为100%。综上,猪流感二价灭活疫苗(H1N1 LN株+H3N2 HLJ株)接种对2021年国内流行的H1N1亚型猪流感病毒攻击产生了较好的交叉保护效果,猪群接种该疫苗后能有效抵抗猪流感流行毒株的攻击。

To evaluate the cross-protection effect of a swine influenza bivalent inactivated vaccine(H1N1 LN strain+H3N2 HLJ strain)against the epidemic H1N1 swine influenza virus strain in China in 2021,ten healthy susceptible 4-week-old piglets were used and randomly assigned to a vaccinated group and a control group,with 5 piglets in each group.Each piglet in the vaccinated group received intramuscularly a dose of 2 ml of the vaccine,followed by a booster immunization with the same dose at 14 days after the first vaccination.The control group did not receive the vaccine.At 28 days after the first immunization,all the piglets were challenged intratracheally with 4.0 ml of A/Swine/Jiangsu/NL33/2021(H1N1)(NL33 strain).Then,the protection effect was assessed based on clinical symptoms,virus isolation from nasal swabs,and lung lesions.The results showed an 80%protection rate in the vaccinated group,and a 100%morbidity rate in the control group.In conclusion,the SIV bivalent inactivated vaccine demonstrated a significant cross-protection against the epidemic H1N1 swine influenza vi⁃rus,effectively enabling vaccinated pigs to resist against the virus challenge.