绵羊肺炎支原体对盘羊杂交羊免疫功能的影响

Effect of Mycoplasma ovipneumoniae on immune function of Argali hybrid sheep

旨在研究绵羊肺炎支原体(Mo)感染盘羊杂交羊后免疫功能的变化。通过酶联免疫吸附法(ELISA)检测免疫球蛋白(Ig)、核因子κB(NF-κB)信号通路相关细胞因子以及CD4浓度和CD8浓度;按照前期建立的绵羊支原体肺炎发病模型的方法,对6只盘羊杂交羊人工感染发病,设为感染组,另外6只盘羊杂交羊注射等量PBS为对照组;在攻毒第0、7、14、21和28天分别采集静脉血,分离血清,检测IgG、IgA、IgM、NF-κB、白细胞介素-1β(IL-1β)、白细胞介素-17(IL-17)、白细胞介素-18(IL-18)、CD4与CD8的浓度。结果:与对照组相比,Mo感染导致盘羊杂交羊血清中IgA与IgM在14 d后显著升高(P<0.05),IgA、IgM、IgG在21 d后极显著升高(P<0.01);NF-κB与IL-1β水平在14 d显著升高(P<0.05),在21、28 d极显著升高(P<0.01);IL-17水平在28 d极显著提升(P<0.01);IL-18水平在21 d后极显著提升(P<0.01);CD4浓度及CD4/CD8比值在14 d显著降低(P<0.01),CD8浓度在14 d后显著升高(P<0.01)。综上,Mo感染盘羊杂交羊后,可刺激宿主的IgA、IgG、IgM水平升高,NF-κB、IL-1β、IL-17和IL-18表达水平上升,CD4浓度降低,CD8浓度升高,使宿主的细胞免疫与体液免疫功能均出现了异常。

In order to explore the changes in immune function of Argali hybrid sheep(AHS)infected with Mycoplasma ovipneumoniae(Mo),enzyme-linked immuno sorbent assay(ELISA)was used in this study to detect the changes.The concentrations of immunoglobulin(Ig),nuclear factorκB(NF-κB)signaling pathway-related cytokines,CD4 and CD8 were detected by ELISA.According to the conditions of the previous model of Mo in sheep,6 AHS were artificially infected as the infection group,and the other 6 AHS were injected with the same amount of PBS as the control group.IgG,IgA,IgM,NF-κB,IL-1β,IL-17,IL-18,CD4 and CD8 in the serum of the sheep were detected at days 0,7,14,21 and 28 after the challenge.The results showed that,compared with the control group,the levels of IgA and IgM in the cross serum of para-sheep were significantly increased at 14 d after Mo infection(P<0.05),and IgA,IgM and IgG were ex⁃tremely significantly increased at 21 d after Mo infection(P<0.01).The levels of NF-κB and IL-1βwere significantly increased at day 14 d(P<0.05),and extremely significantly increased at 21 d and 28 d(P<0.01).The IL-17 level was significantly increased at 28 d(P<0.01).The level of IL-18 was significantly increased at 21 d(P<0.01).The CD4 concentration and the CD4/CD8 ratio were significantly decreased and the CD8 concentration was significantly increased at 14 d(P<0.01).To sum up,Mo infection stimulated the hosts IgA,IgG,IgM levels,raised the NF-κB,IL-1β,IL-17 and IL-18 expression levels,reduced the CD4 concentration,and increased the CD8 concen⁃tration.Abnormal immune function was found in both cellular immunity and humoral immunity in the Argali hybrid sheep infected with Mo.