核壳结构复合材料的制备及抑菌性能研究

Preparation and Bacteriostatic Properties of Core-shell Composite Materials

通过化学还原法,以聚乙烯吡咯烷酮(PVP)为还原剂和保护剂,控制正硅酸乙酯(TEOS)的量制备核壳结构的Ag@SiO_(2)@Ag复合材料。利用SEM、TEM和XRD对核壳结构的Ag@SiO_(2)@Ag复合材料的结构和形貌特征进行表征,结果表明:当加入1 m L的TEOS时,所制得的Ag@SiO_(2)@Ag粒子粒径约为70 nm,且均一性较好,排列整齐。利用紫外-可见分光光度计对所制得的Ag@SiO_(2)@Ag复合材料进行光学性能研究;并研究Ag@SiO_(2)@Ag对大肠杆菌和金黄色葡萄球菌的抑菌效果,结果表明,当加入4 m L的TEOS所制备得到的Ag@SiO_(2)@Ag纳米复合材料,滤纸片浸泡30 min的抑菌效果最佳。TEOS加入的量越多,滤纸片浸泡的时间越长,抑菌圈越明显,抑菌效果越好。在相同条件下,Ag@SiO_(2)@Ag复合材料对金黄色葡萄球菌的抑菌效果更好。

In this paper,the core-shell structured Ag@SiO_(2)@Ag composites were prepared mainly by chemical reduction method using polyvinylpyrrolidone(PVP)as the reducing agent and protective agent by controlling the amount of ethyl orthosilicate(TEOS).The structural and morphological characteristics of core-shell Ag@SiO_(2)@Ag composites were characterized by SEM,TEM and XRD.The results showed that when 1ml of TEOS was added,the prepared Ag@SiO_(2)@Ag particles had a particle size of about 70 nm and good uniformity,with a neat arrangement.The optical properties of the fabricated Ag@SiO_(2)@Ag composites were investigated using a UV-Visible spectrophotometer;and the inhibition effect of Ag@SiO_(2)@Ag on E.coli and S.aureus was investigated,and the results showed that when 4 mL of TEOS was added to the obtained Ag@SiO_(2)@Ag nanocomposites,the best inhibition effect was obtained when the filter paper sheets were soaked for 30 min.The more the amount of TEOS added,the longer the soaking time of the filter paper sheet,the more obvious the inhibition circle and the better the inhibition effect.Under the same conditions,the inhibition effect was better for Staphylococcus aureus.