ε-聚赖氨酸复合水凝胶的制备及抗菌性能

Preparation and Antibacterial Property ofε-Polylysine Composite Hydrogel

利用冻融法制备了负载不同质量分数ε-聚赖氨酸(ε-PL)的聚乙烯醇(PVA)/壳聚糖(CS)复合水凝胶(PCE),并对该复合水凝胶的表面形貌、热稳定性、溶胀性、抗菌性和细胞毒性进行了探讨。结果表明:ε-PL的加入使PCE复合水凝胶的热稳定性增强;随着ε-PL含量的增加,水凝胶的溶胀率呈现出先减小后增大的趋势;此外,当ε-PL的质量分数达到0.6%时,PCE复合水凝胶即可完全杀灭金黄色葡萄球菌和大肠杆菌;PCE复合水凝胶也具有优异的细胞相容性。

Compared to other types of dressings,hydrogels are widely recognized as an optimal choice for wound care due to their superior biocompatibility and high water content.As a novel class of antibacterial materials,hydrogel wound dressings can effectively extend the duration of antibacterial activity and demonstrate promising potential in facilitating tissue regeneration and wound healing.A composite hydrogel(PCE)consisting of polyvinyl alcohol(PVA)and chitosan(CS),loaded with different mass fractions ofε-polylysine(ε-PL),was prepared using a cyclic freeze-thaw method.Subsequently,the surface morphology,thermal stability,swelling property,antibacterial property and cytotoxicity of PCE composite hydrogels were comprehensively discussed.The results showed that there was a hydrogen bond interaction amongε-PL,CS and PVA.The pore size of the composite hydrogel initially decreased and then increased with the increasing ofε-PL mass fraction.The addition ofε-PL improves the thermal stability of the PCE composite hydrogel.As the mass fraction ofε-PL increased,the swelling rate of the composite hydrogel showed a trend of first decreasing and then increasing,which correlated with the changes in pore size.It is worth noting that when the mass fraction ofε-PL reached 0.6%,the swelling rate of the hydrogel reached 651.7%after 24 h,indicating that the hydrogel had excellent swelling performance.This performance facilitates gas exchange,effectively absorbs wound exudate,maintains appropriate humidity conditions for wound healing,and promotes wound healing,and promotes overall recovery.Furthermore,the PCE hydrogel showed complete antibacterial activity against both Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Additionally,the PCE composite hydrogel exhibits good cell compatibility.The prepared PCE composite hydrogel has potential value in wound dressing applications.