SP免疫组化染色检测IGF-1及组织P53蛋白的表达在结直肠腺癌中意义

The significance of SP immunohistochemical staining for detecting the expression of IGF-1 and tissue P53 in colorectal adenocarcinoma

目的探讨链霉菌抗生物素蛋白-过氧化物酶(SP)免疫组化染色检测胰岛素样生长因子-1(IGF-1)及组织P53蛋白的表达在结直肠腺癌中意义。方法选取乌鲁木齐市中医医院2020年1月—2023年12月收治的169例结直肠腺癌患者为恶性组,另选取我院同期收治的169例良性结直肠肿瘤患者为良性组。取手术或活检病理组织应用SP免疫组化染色检测2组肿瘤组织IGF-1及P53蛋白表达水平,对比良性组与恶性组间差异,分析不同临床分期、淋巴结转移情况及组织分化程度患者的IGF-1水平、IGF-1mRNA及P53蛋白阳性率。结果SP免疫组化染色检测IGF-1阳性率、P53蛋白阳性率,恶性组(72.78%、47.93%)均高于良性组(14.79%、6.51%),对比差异有统计学意义(χ^(2)=115.440、χ^(2)=73.180,P<0.05);Ⅳ期患者IGF-1阳性率(96.77%)及P53蛋白阳性率(77.42%)高于Ⅲ期(85.11%、63.83%)、Ⅱ期(62.69%、31.34%)及Ⅰ期患者(45.83%、25.00%),对比差异有统计学意义(χ^(2)=24.860,χ^(2)=28.000,P<0.05),各亚组两两比较差异无统计学意义(IGF-1阳性与阴性,Ⅰ期vsⅢ期,χ^(2)=12.110,P<0.001;Ⅰ期vsⅣ期,χ^(2)=16.060,P<0.001;Ⅱ期vsⅢ期,χ^(2)=6.880,P=0.009;P53蛋白阳性与阴性Ⅰ期vsⅢ期,χ^(2)=9.580,P<0.002;Ⅰ期vsⅣ期,χ^(2)=14.9990,P<0.001;Ⅱ期vsⅢ期,χ^(2)=11.790,P=0.001;);有淋巴结转移患者IGF-1阳性率(85.71%)及P53蛋白阳性率(71.43%)高于无淋巴结转移患者(14.79%、40.94%),对比差异有统计学意义(χ^(2)=4.720、11.740,P<0.05);低分化患者IGF-1阳性率(93.48%)及P53蛋白阳性率(71.74%)高于中分化患者(81.18%、54.12%)、高分化患者(52.63%、31.58%),对比差异有统计学意义(χ^(2)=21.250、13.510,P<0.05)。结论SP免疫组化染色检测提示结直肠腺癌患者IGF-1、P53蛋白阳性率高于良性肿瘤患者,且临床分期高、淋巴结转移与肿瘤组织低分化者的IGF-1、P53蛋白阳性率高,因此IGF-1、P53有望成为结直肠腺癌诊治的重要检测指标。

Objective Exploring the significance of immunohistochemical staining with streptavidin-perosidase(SP)to detect the expression of insulin-like growth factor-1(IGF-1)and tissue P53 in colorectal adenocarcinoma.Methods A total of 169 patients with colorectal adenocarcinoma admitted to a hospital from January 2020 to December 2023 were selected and divided into the malignant group.Other 169 patients with benign colorectal tumors admitted to a hospital during the same period were selected and divided into the benign group.SP immunohistochemistry staining was used to detect and compare the expression levels of IGF-1 and P53 proteins in two groups of tumor tissues obtained in surgery or biopsy.And the IGF-1 levels,IGF-1 mRNA,and P53 positivity rates in patients with different clinical stages,lymph node metastasis,and tissue differentiation levels were compared.Results There was a significant difference in the positive rates of IGF-1mRNA and P53 detected by SP immunohistochemistry staining,and malignant group(72.78%,47.93%)were higher than the benign group(14.79%,6.51%),which were statistically significant(χ^(2)=115.440,73.180,P<0.05).The positive rates of IGF-1 and P53 were 96.77%and 77.42%in stage IV,which were higher than those in Stage III(85.11%,63.83%),II(62.69%,31.34%)and I(45.83%,25.00%),the differences were statistically significant(χ^(2)=24.860,28.000,P<0.05).The expression levels of IGF-1 and P53 in colorectal adenocarcinoma patients with different lymph node metastases showed significant differences,the positive rates of IGF-1(85.71%)and P53(71.43%)in patients with lymph node metastasis were higher than those without lymph node metastasis(14.79%,40.94%),and the differences were statistically significant(χ^(2)=4.720,11.740,P<0.05).There were significant differences in the expression levels of IGF-1 and P53 among patients with colorectal adenocarcinoma of different degrees of differentiation,the positive rates of IGF-1(93.48%)and P53(71.74%)were significantly higher than those of moderately differentiated(81.18%,54.12%)and well differentiated(52.63%,31.58%),and the differences were statistically significant(χ^(2)=21.250,13.510,P<0.05).Conclusions Through SP immunohistochemical staining,it was found that the positivity rates of IGF-1 and P53 in colorectal adenocarcinoma patients were higher than those in benign tumor patients,and those with high the clinical stage,lymph node metastasis,and low differentiation of tumor tissue,had higher the positivity rates of IGF-1 and P53.Therefore,IGF-1 and P53 are expected to become important monitoring indicators for the diagnosis and treatment of colorectal adenocarcinoma.