差分压缩成像型结构光照明超分辨显微技术研究(特邀)

Research on Differential Compressed Imaging-Based Structured Illumination Super-Resolution Microscopy Technique(Invited)

压缩成像型结构光照明超分辨显微(CISIM)技术中不同相移结构的光照明图像的叠加压缩减小了频谱调制成分的占比,影响重构图像质量,针对该问题,发展一种差分压缩成像型结构光照明超分辨显微(DCISIM)技术,利用结构照明压缩图像与宽场图像的参考差分,增大高频图像信息占比,提升重构图像的质量。利用仿真实验验证DCISIM技术的性能优势,同时测试其在不同噪声条件下的成像性能,并简要讨论其技术特点与发展前景。结果表明,DCISIM技术能提升重构图像的精度与细节分辨能力,且能在噪声强度较低的情况下提供较好的超分辨成像效果。

Superposition compression of different phase-shift structured illumination images in compressed imaging-based structured illumination super-resolution microscopy(CISIM)technique reduces the proportion of spectral modulation components,thereby affecting image reconstruction quality.To address this issue,a differential CISIM(DCISIM)technique is developed.This technique utilizes the reference difference between the compressed structured illumination and wide-field images to enhance the information content of high-frequency components and improve the quality of reconstructed images.In this study,the advantages of DCISIM technique are verified through simulation experiments,its performance under different noise levels is tested,and its characteristics and development prospects are briefly discussed.Results indicate that DCISIM technique can improve the accuracy and detailed resolution of reconstructed images and can provide a better super-resolution imaging effect under the condition of low noise intensity.