载脂蛋白E(ApoE)基因单核苷酸多态性与血脂对高强度间歇训练敏感性的关联研究

Apolipoprotein E gene polymorphisms can predict the response to high-intensity interval training

目的探讨载脂蛋白E(ApoE)基因单核苷酸多态性(SNP)与血脂对高强度间歇训练(HIIT)敏感性的关系。方法共招募平原地区汉族230名在校非体育专业大学生(其中男104例,女126例)作为观察对象,所有受试者均进行每周3次、每次28 min、为期12周的HIIT训练。于训练前、训练12周后检测受试者血脂含量,包括甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及总胆固醇(TC)。采集受试者静脉血,提取DNA并使用因美纳(Illumina)基因芯片对质控合格的DNA进行基因分型。使用PLINK(V1.09)软件对所得SNP位点进行质控筛选,并构建线性回归模型,对ApoE基因型与表型进行关联分析。结果(1)通过基因芯片扫描ApoE基因共测得SNP位点21个,经过质控和筛选,共有7个SNPs符合标准,采用PLINK软件构建线性回归模型并去除连锁,发现有1个位点(rs7412)与血脂含量具有极显著相关性。(2)携带ApoE基因rs7412 T等位基因型的TT、CT受试者其TC、LDL-C初始值均显著低于CC基因型受试者(P<0.01),TG、HDL-C初始值在不同基因型受试者间无显著差异。(3)训练干预后受试者LDL-C在不同基因组间有极显著差异(P<0.01),携带T等位基因型的TT、CT受试者经训练后其LDL-C下降幅度显著大于CC基因型受试者。结论ApoE rs7412基因多态性可能与汉族青年TC、LDL-C含量具有显著关联,携带T等位基因型的TT、CT受试者其TC、LDL-C含量均显著低于CC基因型受试者;ApoE基因rs7412多态性可能与HIIT对LDL-C的干预效果有关,携带T等位基因型的TT、CT个体其LDL-C对HIIT训练较敏感。

Objective To seek any relationship between the single nucleotide polymorphisms(SNPs)of the apolipoprotein E(ApoE)gene and blood lipid sensitivity to high-intensity interval training(HIIT).Methods Two hundred and thirty Han Chinese college students(104 males and 126 females)who were not majoring in sports were recruited to perform a 28-minute high-intensity interval workout three times per week for 12 weeks.Before and after the intervention,blood levels of triglycerides(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),and total cholesterol(TC)were measured.The subjects'DNA was genotyped to obtain SNP loci,and a linear regression model was constructed to quantify any association between ApoE genotypes and phenotypes.Results(1)A total of 21 SNP loci were identified associated with ApoE genes,one of which(rs7412)was found to be correlated with the training effect on blood lipids.(2)The initial TT and LDL-C values of carriers of the T allele of gene rs7412(individuals with the CT+TT genotypes)were,on average,significantly lower than those of CC genotype individuals.However,no significant differences in the initial TG and HDL-C values among different genotypes were observed.(3)After the training the average LDL-C levels among the ApoE rs7412 polymorphic groups had changed significantly,with a significantly greater decrease observed among the carriers of the T allele compared with those of CC genotype.Conclusions ApoE polymorphism may be significantly associated with TT and LDL-C values in Han Chinese youth,with carriers of the T allele tending to display lower levels than those of CC genotype.The polymorphism of ApoE gene rs7412 may be related to LDL-C reduction through HIIT,with carriers of the T allele more sensitive to such training.