磁固相萃取-高效液相色谱法测定纺织品中溶剂蓝4残留量

Determination of Solvent Blue 4 in Textiles by HPLC with Magnetic Solid Phase Extraction

本研究建立了一种磁固相萃取结合高效液相色谱法测定纺织品中溶剂蓝4残留量的方法。通过试验,确定了磁固相萃取的最佳前处理条件为:以20 m L甲醇为萃取溶剂,在40℃水浴下超声萃取30 min,然后加入20 mg磁性氧化石墨烯(Fe_(3)O_(4)@GO)作为吸附剂进行吸附,吸附完成后经洗脱、定容、过滤,最后采用高效液相色谱仪进行测定。在优化的仪器参数条件下,溶剂蓝4在0.1~10.00 mg·L^(-1)范围内具有良好的线性关系,检出限和定量限分别为0.2 mg·kg^(-1)和0.5 mg·kg^(-1)。3种添加浓度条件下,溶剂蓝4的回收率在84.1%~93.8%之间,相对标准偏差(Relative Standard Deviations,RSD)(n=6)为2.57%~6.05%。该方法灵敏度高,操作方便,重现性好,满足纺织品中溶剂蓝4残留量的检测。

A method for the determination of solvent blue 4 in textiles sing HPLC coupled with magnetic solid phase extraction was developed.The sample was extracted with 20 m L methanol for 30 min at the temperature of 40℃using ultrasonic extraction.A total of 20 mg of Fe_3O_4@GO was employed as the adsorbent for the magnetic solid phase extraction.Following elution,dilution,and filtration,the solution was qualitatively and quantitatively analyzed by HPLC.Under optimal conditions,a good linear relationship existed in the range of 0.1-10.00 mg·L~(-1),the limits of detection (LOD) and the limits of quantitation (LOQ) were 0.2 and 0.5 mg·kg~(-1).The recoveries were in the range of 84.1%-93.8%,and the relative standard deviations (RSD,n=6) were between 2.57%and 6.05%.The method demonstrates high accuracy,simplicity,and reasonable recovery rates,making it suitable for the detection of solvent blue 4 in textiles.