一种用于单样本多重分析的微流控芯片

A Microfluidic Chip for Single-Sample Multiplex Analysis

呼吸道传染性病原体可能会引起集体爆发性传染病并造成重大经济损失。基于未知感染状态下对样本进行多重分析的需求,利用微纳加工工艺和软光刻技术设计制造了一种聚二甲基硅氧烷(PDMS)和玻璃基底材质的微流控芯片。该芯片利用内部的微柱阵列充当毛细爆破阀实现单一样本的精确均分和并行独立分析。对不同间距微柱的芯片进行进样,结果表明微柱间距为50μm即可确保各均分腔室完全填充,通过对均分腔室形状的优化确保了样本无残留。对单样本中的多种病原体检测结果验证了该芯片的性能。聚合酶链式反应(PCR)荧光检测结果表明,芯片分液的相对标准偏差(RSD)小于5%,并成功实现了12路独立分析。与现有的呼吸道病原体检测微流控芯片相比,该芯片结构简单、操作方便、样本和试剂用量少,减少了人力成本,有一定的临床应用前景。

The respiratory infectious pathogens may lead to outbreaks of communicable diseases and result in significant economic losses.Addressing the multifaceted analytical demands in the context of unknown infection status samples,a microfluidic chip with polydimethylsiloxane(PDMS)and a glass substrate as substrate material was designed and fabricated using micro-nano processing techniques and soft lithography.The chip utilizes an internal array of micropillars acting as capillary burst valves to achieve precise aliquoting and independent analysis of a single sample.Samples were injected to chips with different micropillar spacings and the results reveals that a micropillar spacing of 50μm can ensure complete filling of each subdivided chamber.Furthermore,optimization of the subdivided chamber's geometries ensures absence of sample residues.The chip performances were validated through the detection of multiple pathogens in a single sample.The fluorescence detection results of polymerase chain reaction(PCR)demonstrates that the relative standard deviation(RSD)of the chip's liquid dispensing variation is less than 5%,and 12-channel independent analysis is successfully realiazed.Compared to existing microfluidic chips for respiratory pathogen detection,the chip has simple structure,and is easier to operate,which reducing the required sample and reagent doses,thereby lowering labor costs and showing potential for clinical applications.