细胞球可控制备的微流控芯片

Microfluidic Chip for the Controlled Fabrication of Cell Spheroids

基于热发泡打印原理,采用互补金属氧化物半导体-微电子机械系统(CMOS-MEMS)技术设计了一种可以产生细胞球的微流控芯片,通过调整打印次数控制细胞球的直径。该芯片通过控制底层的CMOS电路驱动电阻发热,从而带动MEMS微结构中的细胞悬液被喷射到指定位置并形成细胞球。实验结果表明:打印过程中,该芯片对CHO细胞活性基本无损伤,且可以制备出平均直径250.4~449.1μm范围内的细胞球,细胞球直径均一性良好,变异系数(CV)为6.17%~10.22%,实现了直径均一的细胞球的可控制备。

Utilizing the thermal bubble jet printing principle,a complementary metal oxide semiconductor-microelectromechanical system(CMOS-MEMS)technology-based microfluidic chip was designed for the fabrication of cell spheroids,and the diameter of the cell spheroids can be controlled by adjusting the number of prints.The chip operates by regulating CMOS circuit at bottom to heat resistors,subsequently actuating the ejection of cell suspension from the MEMS microstructure to designated locations and forming cell spheroids.Experimental results demonstrate minimal impact on CHO cell viability during the printing process.Furthermore,the chip produces cell spheroids within average diameters of 250.4-449.1μm and excellent uniformity with a coefficient of variation(CV)of 6.17%-10.22%,exhibiting the controlled fabrication of cell spheroids with uniform diameters.