从超氧化物歧化酶分析黄芪核桃饮防治痴呆的效果

Analysis of Astragalus Walnut Drink Against Dementia from Superoxide Dismutase

目的分析黄芪核桃饮对阿尔茨海默病模型小鼠(APP/PS1 Tg)的疗效及作用机制。方法将8月龄APP/PS1双转基因雄性小鼠(阿尔茨海默病模型小鼠,APP/PS1 Tg)随机分为阿尔茨海默病模型组(AD组)和黄芪核桃饮组(HH组,中药组),另采用8月龄C57BL/6雄性小鼠作野生对照组(WT组)。对比3组小鼠超氧化物歧化酶(SOD)含量的差异,以及TUNEL在海马区的分布差异。结果①SOD含量方面:治疗后,AD组与WT组比较,同月龄小鼠均有显著差异;HH组8月龄小鼠与WT组有显著差异,而与AD组无明显差异;HH组10月龄小鼠与WT组无显著差异,而与AD组差异显著;②TUNEL在海马区的分布上,治疗后HH组与WT组无差异,与AD组有显著差异。结论经黄芪核桃饮治疗后APP/PS1小鼠认知功能改善与其抗氧化抗凋亡能力有关。

Objective To analyze the efficacy and mechanism of action of Astragalus walnut drink on Alzheimer’s disease model mice(APP/PS1 Tg).Methods 8-month-old APP/PS1 double transgenic male mice(Alzheimer’s disease model mice,APP/PS1 Tg)were randomly divided into the Alzheimer’s disease model group and the Astragalus walnut drink group(traditional Chinese medicine group),and 8-month-old C57BL/6 male mice were used in the wild control group.Differences in superoxide dismutase content and distribution of TUNEL in the hippocampus were compared among the three groups of mice.Results①There was a difference in SOD between the Chinese medicine group and the wild group after treatment,and there was a significant difference with the Alzheimer’s disease model group;②About the distribution of TUNEL in the hippocampus,there was no difference between the Chinese medicine group and the wild group after treatment,and there was a significant difference with the Alzheimer’s disease model group.Conclusion The improvement of cognitive function in APP/PS1 mice treated with Astragalus Walnut Drink is related to its antioxidant capacity.