日粮添加复合益生菌对英国短毛猫粪便微生物区系的影响

Effect of dietary compound probiotics on fecal microflora of British shorthair cats

研究旨在探讨日粮中添加复合益生菌对英国短毛猫粪便微生物区系的影响。试验选取英国短毛猫24只,分别在饲喂复合益生菌前、饲喂10、20 d时收集新鲜粪便样本,利用16S rDNA测序技术分析粪便菌群多样性、种属组成及菌群对宿主生理功能的影响。结果显示,与饲喂前相比,饲喂复合益生菌后平均体重和平均日采食量无变化,粪便评分无差异。但饲喂复合益生菌后,粪便菌群多样性明显改善,且前期变化较大、后期趋于稳定。粪便中乳杆菌属、双歧杆菌属、小杆菌属相对丰度升高,厚壁菌门/拟杆菌门比值升高。生理功能互作预测发现,复合益生菌促进了短链脂肪酸生成、葡萄糖酸铜降解、肌醇降解等生理过程,减弱了尿素循环等过程。研究表明,日粮中添加复合益生菌维持了英国短毛猫肠道菌群平衡,促进了益生菌的增殖,有利于猫肠道健康。

The study aimed to investigate the effects of dietary supplementation with compound probiotics on the fecal microbiota of British shorthair cats.Twenty-four British Shorthair cats were selected,and fresh fecal samples were collected before supplementation,10 days after,and 20 days after the supplementation with the compound probiotics.Fecal bacterial diversity,composition,and the impact on the host's physiological functions were analyzed using 16S rDNA sequencing technology.The results showed that compared to before supplementation,there were no changes in the average body weight and daily food intake of the cats,and no difference in fecal scores.However,after supplementation with the compound probiotics,the diversity of the fecal microbiota in cats significantly improved,with greater changes in the early stage and a tendency to stabilize in the later stage.The relative abundance of Lactobacillus,Bifidobacterium,and Enterococcus in the feces increased,and the Firmicutes/Bacteroidetes ratio increased.Predictions of physiological function interactions revealed that the addition of the probiotic mixture promoted physiological processes such as short-chain fatty acid production,glucuronic acid degradation,and inositol degradation in the host,while weakening processes such as the urea cycle.The study indicates that the addition of the compound probiotics to the diet maintained the balance of the gut microbiota,promoted the proliferation of probiotics,and is beneficial to the intestinal health of the British shorthair cats.