采用代谢组学方法分析高原肺水肿患者血浆小分子代谢产物的初步研究

Problems and countermeasures in the ESG in formation disclosure of the Stationery Industry

目的 低压低氧、寒冷、强紫外线、干燥是高原特有环境。在急进海拔2500 m以上的环境下极易发生肺动脉压增高、进而增加了肺血容量,导致毛细血管内液体渗出至肺间质及肺泡,肺泡和间质水肿、肺泡内纤维蛋白渗出和透明膜形成、肺毛细血管极度扩张和充血及肺泡清除能力降低,从而导致高原肺水肿(High Altitude Pulmonary Edema, HAPE)。当前,对于高原肺水肿(HAPE)的诊断,尚未发现一个完美的生物标志物,这促使科研人员不断探索和尝试各种创新方法,以期发现新的、有效的生物学标志物。这种探索已成为高原肺水肿研究领域的热门趋势。该研究采用代谢组学技术对高原肺水肿患者和正常对照血浆中的小分子代谢物质进行分析,以寻求潜在的高原肺水肿标志物。方法 运用液相色谱串联质谱法(LC-MS/MS)对30例高原肺水肿患者与30例急进藏正常对照的血浆样本进行代谢组学分析,并利用正交偏最小二乘法(OPLS-DA)构建模型;通过双样本检验,挖掘两组血浆样本中的差异代谢物。结果 血浆内共鉴定出3184种代谢产物,并且多变量分析揭示两者在代谢物水平上存在显著的差异性,基于统计检验确定了143种与血浆差异相关的代谢产物。结论 利用代谢组学方法预期发现敏感性、特异性较高的生物标志物,这将为高原肺水肿的诊断和治疗带来革命性的进展。

Objective Low pressure,low oxygen,coldness,strong ultraviolet rays,and dryness are the unique environments of the plateau.In the environment of rapid entry into the altitude more than 2500 m,the pulmonary arteryi pressure is easy to increase,which in turn increases pulmonary blood volume,leading to the exudation offluid in the capillaries to the pulmonary interstitium and alveoli,alveolar and interstitial edema,intraalveolar fibrin exudation and hyaline membrane formation,extreme dilatation and congestion of pulmonary capillaries and a reduced alveolar clearanceability,resulting in high-altitude pulmonary edema(HAPE).At present,there is no perfect biomarker for the diagnosis of HAPE,which prompts researchers to continue to explore and try various innovative methods in order to discover new and effective biomarkers.This exploration has become a popular trend in the field of HAPE research.In this study,metabolomics technology was used to analyze the small molecule metabolites in plasma of patients with HAPE and normal controls,in order to seek potential markers of HAPE.Methods Liquid chromatography tandem mass spectrometry(LC-MS/MS)was used to analyze the plasma samples of 30 patients with HAPE and 30 normal controls who rushed to Xizang,and the orthogonal partial least squares(OPLS-DA)method was used to construct the model.The differential metabolites in the plasma samples of the two groups were explored by the two-sample test.Results A total of 3184 metabolites were identified in plasma,and multivariate analysis revealed significant differences in metabolite levels between the two groups,and 143 metabolites related to plasma differences were identified based on statistical tests.Conclusion Metabolomics is expected to detect sensitive and specific biomarkers,which will revolutionize the diagnosis and treatment of HAPE.