卵泡抑素样蛋白1与室性心律失常的相关性研究

Correlation between follistatin-like protein 1 and ventricular arrhythmia:report of 107 cases

目的探讨卵泡抑素样蛋白1(follistatin-like protein 1,FSTL1)水平与室性心律失常疾病程度的相关性。方法选取2019年10月至2020年9月于云南省第一人民医院老年医学科治疗的患者107例,根据Lown氏分级法分为对照组40例、偶发室性期前收缩组35例、频发室性期前收缩组20例和室性心动过速组12例。采用酶联免疫吸附测定法测定患者血浆FSTL1水平,运用实时荧光定量聚合酶链反应检测各组FSTL1 mRNA表达水平。比较4组心电图指标、血浆FSTL1、FSTL1 mRNA表达水平。结果频发室性期前收缩组和室性心动过速组FSTL1水平显著高于对照组,差异有统计学意义[(39.62±14.29)ng/L,(50.66±21.37)ng/L vs(24.58±10.15)ng/L,P<0.05];频发室性期前收缩组和室性心动过速组FSTL1水平显著高于偶发室性期前收缩组,差异有统计学意义[(39.62±14.29)ng/L,(50.66±21.37)ng/L vs(31.83±9.54)ng/L,P<0.05];室性心动过速组FSTL1水平显著高于频发室性期前收缩组,差异有统计学意义[(50.66±21.37)ng/L vs(39.62±14.29)ng/L,P<0.05]。频发室性期前收缩组和室性心动过速组FSTL1 mRNA表达显著高于对照组和偶发室性期前收缩组,差异有统计学意义(P<0.05);室性心动过速组FSTL1 mRNA表达显著高于频发室性期前收缩组,差异有统计学意义(P<0.05)。结论在一定程度上,患者血液中FSTL1水平与室性心律失常疾病程度存在正相关关系,有望成为疾病预警的新靶点。

Objective To reveal the correlation between follistatin-like protein 1(FSTL1)level and severity of ventricular arrhythmia.Methods A total of 107 patients treated in our department from October 2019 to September 2020 were recruited and divided into control group(40 cases),accidental premature ventricular contraction(PVC)group(35 cases),frequent PVC group(20 cases)and ventricular tachycardia(VT)group(12 cases)according to the Lown's grading.The blood content of FSTL1 was determined by ELISA,and its expression level was detected by RT-qPCR.Electrocardiographic indicators,blood FSTL1 content and relative level of FSTL1 were compared among the four groups.Results The FSTL1 content was significantly higher in the frequent PVC group and the VT group than the control group[(39.62±14.29)ng/L and(50.66±21.37)ng/L vs(24.58±10.15)ng/L,P<0.05],so was the content in the frequent PVC group and the VT group than the accidental PVC group[(39.62±14.29)ng/L and(50.66±21.37)ng/L vs(31.83±9.54)ng/L,P<0.05].The VT group had significantly higher FSTL1 than the frequent PVC group[(50.66±21.37)ng/L vs(39.62±14.29)ng/L,P<0.05].The mRNA level of FSTL1 was significantly higher in the frequent PVC group and the VT group than the control group and the accidental PVC group(P<0.05),and in the VT group than the frequent PVC group(P<0.05).Conclusion To a certain extent,the blood FSTL1 content is positively correlated with the severity of ventricular arrhythmia,which may be a new target for disease warming.