玉米醇溶蛋白-葡聚糖-叶黄素微胶囊的制备及性能研究

Study on Preparation and Properties of Zein-Dextran-Lutein Microcapsules

叶黄素对光、热敏感,导致其稳定性差,为解决此问题,对叶黄素进行微胶囊化处理。文章以叶黄素为芯材,玉米醇溶蛋白、葡聚糖为壁材,采用反溶剂沉淀法对叶黄素进行包埋,在单因素试验的基础上采用响应面法优化叶黄素微胶囊的制备工艺,对其进行表征并测定其贮藏稳定性和抗氧化性。结果表明,叶黄素微胶囊的最佳制备条件为乙醇浓度84%、溶剂与反溶剂体积比1∶2、反应温度39℃、玉米醇溶蛋白浓度4.0 mg/mL、葡聚糖浓度1.0 mg/mL,此时包埋率为90.53%,载药率为8.23%。包埋后叶黄素的饱和溶出度提高了4.84倍,贮藏6周后,叶黄素微胶囊在光照条件下的保留率高于叶黄素35.20%,在25℃下叶黄素微胶囊的保留率高于叶黄素23.11%,在4℃下叶黄素微胶囊的保留率高于叶黄素16.31%,在-20℃下叶黄素微胶囊的保留率仅下降1.91%。叶黄素微胶囊对DPPH、ABTS+自由基的抗氧化活性高于未包埋的叶黄素。该研究结果可为叶黄素微胶囊作为新型添加剂在工业领域的应用提供理论参考。

Lutein is sensitive to light and heat,which leads to its poor stability.In order to solve this problem,lutein is microencapsulated.In this paper,with lutein as the core material and zein and dextran as the wall materials,lutein is embedded by antisolvent precipitation method.The preparation process of lutein microcapsules is optimized by response surface method on the basis of single factor test,and they are characterized and their storage stability and antioxidant activity are characterized.The results show that the optimal preparation conditions of lutein microcapsules are ethanol concentration of 84%,the volume ratio of solvent to antisolvent of 1∶2,reaction temperature of 39℃,zein concentration of 4.0 mg/mL and dextran concentration of 1.0 mg/mL.At this time,the embedding rate is 90.53%,the drug loading rate is 8.23%.After embedding,the saturation dissolution rate of lutein increases by 4.84 times.After six weeks of storage,the retention rate of lutein microcapsules is 35.20%higher than that of lutein under light conditions,23.11%higher than that of lutein at 25℃,and 16.31%higher than that of lutein at 4℃.At-20℃,the retention rate of lutein microcapsules only decreases by 1.91%.The antioxidant activity of lutein microcapsules against DPPH and ABTS+free radicals is higher than that of unembedded lutein.The research results can provide theoretical references for the application of lutein microcapsules as new additives in the industrial field.