适宜贵州省安龙县夏季栽培香菇菌株的筛选

Selection of suitable Lentinula edodel strains for summer cultivation in Anlong,Guizhou province

为了筛选出安龙县夏季适合栽培的香菇菌株,以安龙县各香菇种植企业收集的6株香菇菌株为试验材料,比较6株香菇的菌丝生长速度、拮抗反应、基因序列、子实体单棒产量,并构建系统发育树。结果表明,AL3菌株的菌丝生长速度较快,平均生长速度为4.51 mm·d^(-1),显著高于其他菌株,其次是AL6菌株,平均生长速度为4.37 mm·d^(-1),AL1和AL6菌株与其他菌株之间拮抗现象明显,其余菌株拮抗不显著或无拮抗;ITS序列比对及系统发育分析将6株香菇菌株聚为香菇属进化的3个大类,2个不同菌株及4个不同亚种;AL4菌株单棒产量最高,为0.58 kg,其次是AL6菌株,单棒产量为0.47 kg。综合各试验结果分析得出,6株菌株中,AL4、AL6和AL3菌株较适合在安龙县夏季栽培,研究结果可为安龙县夏季适栽香菇种质资源的筛选提供理论依据。

In order to select suitable Lentinula edodels strains for summer cultivation in Anlong county,six L.edodels strains collected from various L.edodels strains growing enterprises in Anlong county were used as test materials,and the mycelial growth rate,antagonistic response,gene sequence,and single rod yield of the substrate of the six L.edodels strains were compared,and phylogenetic tree was constructed.The results showed that among the six L.edodels strains,the growth rate of the AL3 strain was faster,with an average growth rate of 4.51 mm·d^(-1),which was significantly higher than that of the other strains,followed by that of the AL6 strain,with an average growth rate of 4.37 mm·d^(-1);antagonism between the AL1 and AL6 strains and the other strains was obvious,and the rest of the strains had no antagonism that was not significant or antagonistic;ITS sequence alignment and phylogenetic analyses placed the six L.edodels strains into three major evolutionary categories of the Lentinula,the six strains were categorized into two homozygous strains and four different subspecies.AL4 strain had the highest single rod yield of 0.58 kg,followed by AL6 strain with single rod yield of 0.47 kg.The results of the experiments showed that among the six L.edodels strains,AL4,AL6 and AL3 strains were more suitable for summer cultivation in Anlong country,which provided theoretical support for the selection of suitable L.edodels strains germplasm resources for summer cultivation in Anlong county.