线叶金雀花黄酮动态超高压微射流均质辅助乙醇提取及其调节血糖作用研究

Dynamic Ultra high Pressure Micro Jet Homogenization Assisted Ethanol Extraction of Rooibos Flavonoid and Its Regulation of Blood Glucose

以线叶金雀花为原料,采用动态超高压微射流均质辅助乙醇提取其黄酮,经响应面优化提取工艺,同时研究其降血糖作用。结果表明,最佳提取工艺条件为液固比29:1(mL/g)、微射流压力147 MPa、提取时间63 min,在此条件下,线叶金雀花黄酮得率为2.86%。小鼠降血糖试验结果表明,线叶金雀花黄酮能够降低小鼠体质量和血糖值,提高小鼠糖耐量,降低小鼠血清中总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)和低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol,LDL-C),增加高密度脂蛋白胆固醇(High-density lipoprotein cholesterol,HDL-C),降低小鼠体内丙二醛(Malondialdehyde,MDA)含量,提高过氧化氢酶(Catalase,CAT)、谷胱甘肽(Glutathione,GSH)和超氧化物歧化酶(Superoxide dismutase,SOD)的活性。这表明线叶金雀花黄酮具有调节血糖作用,同时还可发挥调节血脂和抗氧化活性作用。研究结果可为线叶金雀花黄酮的提取及产品应用开发提供参考。

Using rooibos as raw material,its flavonoids were extracted by dynamic ultra high pressure micro jet homogenization assisted ethanol method.The extraction process was optimized by response surface methodology,and its hypoglycemic effects were studied.The results showed that the optimal extraction process conditions were:liquid-to-solid ratio was 29:1(mL/g),micro jet pressure was 147 MPa,and extraction time was 63 minutes.Under these conditions,the yield of rooibos flavonoids was 2.86%.The results of the hypoglycemic effect test in mice showed that rooibos flavonoids could reduce body weight and blood glucose levels,improve glucose tolerance,reduce total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)in mouse serum,increase high-density lipoprotein cholesterol(HDL-C),reduce malondialdehyde(MDA)content,increase the activity of catalase(CAT),glutathione(GSH),and superoxide dismutase(SOD)in mice.This indicates that rooibos flavonoids have the effect of regulating blood sugar,as well as regulating blood lipids and antioxidant activity.This experiment can provide a reference for the extraction and product application development of rooibos flavonoid.