玉米纹枯病菌菌核形成相关基因CYP450表达特性分析

Analysis on the expression characteristics of CYP450 related to sclerotium formation in Rhizoctonia solani

为了从立枯丝核菌菌核形成探寻纹枯病防治方法,试验从田间采集玉米纹枯病发病植株,分离纯化立枯丝核菌菌株。使用生物信息学分析软件对CYP450基因的结构与功能进行分析,可知CYP450可能是一种亲水性蛋白且不存在跨膜序列,由397个氨基酸共同编码,含有信号肽。利用实时荧光定量PCR技术,对立枯丝核菌CYP450基因菌核形成过程中基因表达量变化进行研究,探寻CYP450基因在菌核形成过程中的作用。分析结果显示CYP450基因在菌核形成过程中表达量有显著差异,其中在菌丝时期表达量低,菌核形成后表达量有逐渐增高趋势,即在立枯丝核菌菌核形成过程中,CYP450可能参与了玉米纹枯病菌菌核的形成,该结果为玉米纹枯病的防治及抗病育种提供理论依据。

In order to explore the prevention and treatment of Rhizoctonia solani from the formation of sclerotinia sclerotium,the infected plants of Rhizoctonia solani were collected from the field and the strains were isolated and purified.The structure and function of CYP450 gene were analyzed by bioinformatics analysis software.It was concluded that CYP450 may be a hydrophilic protein without a transmembrane sequence,encoded by 397 amino acids and containing signal peptides.The expression of CYP450 gene during sclerotium formation of Rhizoctonia solani was studied by real-time quantitative PCR to explore the role of CYP450 in sclerotium formation.The results showed that the expression level of CPY450 gene was significantly different in the process of sclerotium formation,and the expression level of CPY450 gene was low in the mycelial stage.The expression of sclerotium gradually increased after sclerotia formation,that is CYP450 may be involved in the sclerotium formation of Rhizoctonia solani.The results provide theoretical basis for the prevention control of maize sheath blight and breeding of disease resistant.