暴雨径流对水源水库细菌群落结构和代谢活性及水质的影响

Effects of Storm Runoff on Bacterial Community Structure and Metabolic Activity and Water Quality in a Drinking Water Reservoir

为探究汛期暴雨径流入库对水库细菌代谢活性及水质的影响,以金盆水库为研究地点,采用三维荧光光谱-平行因子分析法、高通量DNA测序技术和Biolog技术分析金盆水库径流3个时期(径流前、径流中和径流后一个月)的有机质、细菌群落组成和微生物代谢活性变化.结果表明,径流入库后,水体温度降低,水体中层溶解氧显著提高,形成中层水体好氧低温,底层厌氧低温的状态.总氮、总磷、有机质浓度和浊度在径流期间都大幅提升,一个月后有所下降,但没有恢复到径流前的浓度.径流期间,变形菌门和拟杆菌门占比显著提高,二者相对丰度之和占细菌群落的63.6%.细菌代谢活性也高于径流前,且在一个月后,细菌种群结构并没有恢复到径流前,细菌代谢活性仍保持较高水平.

To explore the influence of storm runoff on bacterial metabolic activity and water quality in Jinpen Reservoir,three-dimensional fluorescence spectroscopy parallel factor analysis,high-throughput DNA sequencing technology,and Biolog technology were used to analyze variations in the components of dissolved organic matter,bacterial community composition,and microbial metabolic activity during three periods(before,during,and one month after runoff).The results showed that after storm runoff into the reservoir,the water temperature decreased,and dissolved oxygen in the middle of the water column increased significantly.Finally,the aerobic and low-temperature state in the middle water and anaerobic and low-temperature state in the bottom water was formed.Simultaneously,the concentration of total nitrogen,total phosphorus,organic matter,and turbidity increased significantly.After one month of runoff,the concentration of pollutant decreased,however,was still higher than that before runoff.Further,during the runoff period,the proportion of Proteobacteria and Bacteroidetes increased significantly and the sum of their relative abundance accounted for 63.6%of the bacterial community.The bacterial metabolic activity was also higher than that before.One month after runoff,the bacterial population structure did not recover and the bacterial metabolic activity still remained at a high level.