北京地区老年人群凝血酶-抗凝血酶复合物和纤溶酶-α2纤溶酶抑制剂复合物参考区间的研究

Reference intervals of thrombin-antithrombin complex and plasmin-α2-antiplasmin complex in the elderly population in Beijing

目的探讨凝血酶-抗凝血酶复合物(TAT)和纤溶酶-α2纤溶酶抑制剂复合物(PIC)在北京地区表观健康老年人群中的水平,并建立参考区间。方法选取2023年1月1日至2023年9月30日首都医科大学附属北京安贞医院健康管理中心体检的表观健康老年人839例,其中男418例(60~<70岁141例、70~<80岁135例、≥80岁142例),女421例(60~<70岁145例、70~<80岁140例、≥80岁136例)。采用广州万孚生物全自动化学发光免疫分析仪Shine i2900及配套试剂检测TAT和PIC水平,分析其在不同性别和年龄组人群中的分布情况,并建立参考区间。结果TAT在各年龄段男性中的水平均低于女性(P<0.01),60~<70岁与70~<80岁中,男性组内和女性组内的结果比较差异无统计学意义(P>0.05),60~<80岁与≥80岁中,男性组内和女性组内的结果比较差异有统计学意义(P<0.01),且随年龄增长呈上升趋势。PIC水平在男性组与女性比较组差异无统计学意义(P>0.05),在各年龄段,男性组内和女性组内的结果比较差异有统计学意义(P<0.01),且总体水平随年龄增长呈上升趋势。TAT参考区间:男性60~<80岁为0.69~2.42 ng/mL,男性≥80岁为0.87~3.71 ng/mL,女性60~<80岁为0.67~2.82 ng/mL,女性≥80岁为1.00~3.66 ng/mL;PIC参考区间:60~<70岁为0.10~0.89μg/mL,70~<80岁为0.12~0.99μg/mL,≥80岁为0.21~1.06μg/mL。结论健康老年人的TAT和PIC水平在不同性别和年龄段中存在差异,该研究初步建立了北京地区表观健康老年人群的TAT和PIC参考区间。

Objective To explore the levels of thrombin-antithrombin complex(TAT) and plasmin-α2-antiplasmin complex(PIC) in the apparently healthy elderly population in Beijing,and to establish the reference intervals.Methods A total of 839 elderly individuals,including 418 males(60-<70 year-old 141 cases,70-<80 year-old 135 cases,≥80 year-old 142 cases) and 421 females(60-<70 year-old 145 cases,70-<80 year-old 140 cases,≥80 year-old 136 cases),who underwent physical examinations at the Health Management Center of Beijing Anzhen Hospital,Capital Medical University from January 1,2023 to September 30,2023,were selected.The levels of TAT and PIC in plasma were detected using Shine i2900(Guangzhou Wondfo Biotech Co.,Ltd.) automated chemiluminescence immune detection system and its supporting reagents.The distribution among different gender and age groups was analyzed,and reference intervals were established.Results The levels of TAT in males across all age groups were lower than those in females(P<0.01).There was no difference in the comparison results between the 60-<70 year-old and the 70-<80 year-old within the male and female groups,respectively.The differences between the ages of 60-<80 year-old and ≥80 year-old in both gender groups were statistically significant(P<0.01),and the levels showed an upward trend with age.There was no difference in the level of PIC between the male and female groups(P>0.05),while there were differences in all age groups within the two gender groups(P<0.01),and the trend was increasing with age.TAT:for males aged 60-<80 and ≥ 80,the reference intervals were 0.69-2.42 ng/mL and 0.87-3.71 ng/mL,respectively.For females aged 60-<80 and ≥ 80,the reference intervals were 0.67-2.82 ng/mL and 1.00-3.66 ng/mL,respectively.PIC:for people aged 60-<70,70-<80,and ≥ 80 were 0.10-0.89 μg/mL,0.12-0.99 μg/mL,and 0.21-1.06 μg/mL,respectively.Conclusion There are differences in TAT and PIC among different gender and age groups in the elderly population with apparent health.This study successfully established reference interval for TAT and PIC in the elderly population with apparent health in Beijing.