吡喃衍生物传感明胶双通道指示鱼肉新鲜度

Pyran Derivative Sensing Gelatin Dual-Channel Indication of Fish Freshness

以苯并吡喃为荧光团,通过引入吸电子基三氰基呋喃延长共轭体系,设计合成了胺类敏感型荧光探针CHM。该探针在EtOH/H2O(5/5,V/V)体系中对11种胺有荧光增强响应,具有较大的斯托克斯位移(120 nm),较低的检测限(腐胺,1.3μmol/L),并可在活细胞中对腐胺荧光成像。将CHM负载到明胶上,制备的传感明胶CHMGL对挥发性胺的颜色变化明显,可通过比色和荧光双通道对三文鱼鱼肉新鲜度进行实时监测,从而准确判断鱼肉的鲜度。

In this paper,an amine-sensitive fluorescent probe CHM was designed and synthesized using benzopyran as a fluorophore by introducing an electron-withdrawing group tricyanofuran to extended conjugated system.The probe can recognize 11 amines with an off-on fluorescence response,a large Stokes shift(120 nm),a low detection limit(putrescine,1.3μmol/L)in EtOH/H2O(5/5,V/V)solution,and can imaging for putrescine in living cells.Probe CHM is loaded onto the gelatin to obtain sensing gelatin CHMGL.CHMGL shows significant color changes to volatile amines,which can be used to monitor the freshness of salmon fish in real time through colorimetric and fluorescence dual-channels,and can accurately judge the freshness of fish.