桃树细菌性穿孔病生防菌的筛选鉴定及防治

Screening Identification of Biocontrol Bacteria and Control of Peach Bacterial Shot Hole

为了获得对桃细菌性穿孔病(peach bacterial shot hole)具有较好防治效果的生防菌株,通过平板对峙法从桃树根际土壤中筛选拮抗菌株并进行防治效果的测定。结果表明:共有198株细菌菌株被分离纯化出来,其中13株对桃细菌性穿孔病表现出抑制效果,菌株8号的抑制效果最为显著,其抑菌直径为30.8 mm;通过形态学及分子鉴定,并建立系统发育树,鉴定菌株8号为耐盐芽孢杆菌(Bacillus halotolerans);离体接种及田间防治试验表明,相较于对照组,菌株8号能较好抑制病害的扩散,并减缓发病率的增加。综上,菌株8号对于桃细菌性穿孔病具有很好的生防潜力,可为该病害的生防菌的开发及应用提供依据。

Antagonistic strains were screened from peach rhizosphere soil by agar disk method and the control effect was determined so as to obtain biocontrol strains with good control effect on peach bacterial shot hole.The results show that a total of 198 bacterial strains are isolated and purified,among which 13 strains show inhibitory effect on peach bacterial perforation disease.The inhibitory effect of strain No.8 is the most significant,and its inhibitory diameter is 30.8 mm.Strain 8 is identified as salt-tolerant Bacillus halotolerans by morphological and molecular identification and phylogenetic tree is established.In vitro inoculation and field control experiments show that strain 8 could better inhibit the spread of the disease and slow down the increase of morbidity compared with the control group.In summary,strain 8 has a good biocontrol potential for peach bacterial perforation disease,and can provide a basis for the development and application of biocontrol bacteria for this disease.