乳腺癌患者血清HOTAIR、miR-331-3p水平预测术后复发的风险模型构建与验证

Construction and Validation of Serum HOTAIR and miR-331-3p Levels to Predict the Risk of Postoperative Recurrence in Patients with Breast Cancer

目的探讨乳腺癌患者血清HOX转录反义RNA(HOTAIR)、miR-331-3p水平与术后复发的关系,并构建风险模型。方法选取2019年6月至2021年6月我院乳腺癌手术患者108例为研究组,另选取同期健康体检者45例为对照组。比较两组血清HOTAIR、miR-331-3p水平,统计乳腺癌患者术后2年复发情况,采用Cox回归分析乳腺癌患者术后复发影响因素,建立列线图预测模型并验证。结果研究组血清HOTAIR、miR-331-3p水平高于对照组(P<0.05);Cox回归模型分析结果显示,临床分期Ⅲ期、人表皮生长因子受体2(HER2)阳性、血清HOTAIR与miR-331-3p高表达均为乳腺癌患者术后复发独立危险因素,雌激素受体(ER)表达阳性、孕激素受体(PR)表达阳性、术后化疗、术后内分泌治疗是保护因素(P<0.05);列线图模型预测的准确性和有效性较好。结论临床分期Ⅲ期、HER2阳性、血清HOTAIR与miR-331-3p高表达均为乳腺癌患者术后复发独立危险因素,ER阳性、PR阳性、术后化疗、术后内分泌治疗是保护因素,且据此建立的列线图模型具有较高的预测价值。

Objective To investigate the relationship between serum HOX transcribed antisense RNA(HOTAIR)and miR-331-3p levels and postoperative recurrence in patients with breast cancer,and to establish a risk model.Methods 108 patients who underwent breast cancer surgery in our hospital from June 2019 to June 2021 were selected as study group,and 45 healthy patients during the same period were selected as control group.Serum HOTAIR and miR-331-3p were compared,and the recurrence of breast cancer patients 2 years after surgery was statistically analyzed.Cox regression was used to analyze the influencing factors of postoperative recurrence of breast cancer patients,and a nomogram prediction model was established and verified.Results Serum HOTAIR and miR-331-3p in study group were higher than those in control group(P<0.05).Cox regression model analysis showed that clinical stageⅢ,positive human epidermal growth factor receptor 2(HER2),high expression of serum HOTAIR and miR-331-3p were independent risk factors for postoperative recurrence of breast cancer patients.Positive expression of estrogen receptor(ER),progesterone receptor(PR),postoperative chemotherapy and postoperative endocrine therapy were protective factors(P<0.05).The accuracy and effectiveness of the nomogram model were good.Conclusion Clinical stageⅢ,HER2 positivity,and high expression of serum HOTAIR and miR-331-3p were all independent risk factors for postoperative recurrence in breast cancer patients,and ER positivity,PR positivity,postoperative chemotherapy,and postoperative endocrine therapy were protective factors,and the columnar graphical model based on this was of high predictive value.