滇黄精多糖对肥胖小鼠脂代谢紊乱及脑功能损伤的改善作用

Ameliorative Effects of Polygonatum kingianum Polysaccharides on Lipid Metabolism Disorders and Brain Function Impairments in Obese Mice

为研究滇黄精多糖(Polygonatum kingianum polysaccharides,PKP)对肥胖小鼠脂代谢紊乱及脑功能损伤的作用效果,采用酶法辅助提取的PKP干预肥胖小鼠12周,观察小鼠体质量和血脂变化以及肝脏和脂肪组织的病理形态变化,并通过行为学检测小鼠自主活动性和焦虑样行为变化,进一步检测肝脏、脂肪、血清和脑组织炎症水平及脑组织病理形态变化。结果表明:PKP(1500 mg/kg)减少高脂饮食诱导肥胖小鼠的体质量增量29.6%,有效改善血脂水平异常及肝脏和脂肪的组织形态,并促进肝脏过氧化物酶体增殖物激活受体α(46.0%)和脂肪解偶联蛋白1表达(59.0%),同时明显增加肥胖小鼠的自主活动性并改善焦虑行为;肝脏和脂肪F4/80表达水平分别减少55.0%和67.2%,血清中促炎因子白细胞介素1β、白细胞介素6和肿瘤坏死因子α水平分别减少22.9%、44.6%和7.8%,而抗炎因子白细胞介素10水平增加27.1%;大脑海马和皮层区离子钙结合衔接分子1表达水平分别减少16.0%和28.6%,改善了海马和皮层区域结构损伤。PKP有效改善了肥胖小鼠脂代谢紊乱及脑功能损伤,为其作为抗肥胖及改善肥胖诱导脑损伤的功能性食品配料提供了科学依据。

To study the effects of Polygonatum kingianum polysaccharides(PKP)on lipid metabolism disorders and brain function impairments in obese mice,the obese mice were treated with PKP,prepared by enzyme-assisted extraction,for 12 weeks.Changes in body mass and blood lipids,as well as pathological changes in liver and adipose tissue were observed.Behavioral changes were detected to assess the autonomic activity and anxiety-like behavior of mice,and inflammation levels in liver,adipose tissue,serum,and brain as well as pathomorphological changes in brain were detected.The results showed that PKP(1500 mg/kg)reduced body mass gain by 29.6%in obese mice,significantly alleviated dyslipidemia and pathological changes in liver and adipose tissue,and promoted the expression of peroxisome proliferator-activated receptorsα(PPARα)in liver(46.0%)and the expression of uncoupling protein 1 in adipose tissue(59.0%).PKP significantly increased autonomic activity and suppressed anxiety-like behavior in obese mice.Furthermore,PKP reduced the expression of F4/80 in liver and adipose tissue by 55.0%and 67.2%,respectively,and the serum levels of pro-inflammatory cytokines in including interleukin 1βby 22.9%,interleukin 6 by 44.6%,and tumor necrosis factorαby 7.8%,but increased the serum level of anti-inflammatory cytokine interleukin 10 by 27.1%.Furthermore,PKP reduced the expression levels of ionized calcium binding adaptor molecule 1 in the hippocampus and cerebral cortex by 16.0%and 28.6%,respectively,and inhibited structural damages of the hippocampus and cerebral cortex.PKP mitigate obesity-induced lipid disorders and brain damages,indicating their potential as a functional food ingredient for obesity and suppressing obesity-induced brain damage.