牡丹花中不同形态酚类化合物及其抗氧化和α-葡萄糖苷酶抑制活性

Composition of Free and Bound Phenolics from Paeonia suffruticosa Andr.Flowers and Their Antioxidant andα-Glucosidase Inhibitory Activities

对丹凤和香玉两种牡丹花中的游离酚、酯键合态酚、糖苷键合态酚和不溶性结合态酚进行提取,测定不同形态酚类化合物中总酚、总黄酮含量及其主要化合物组成和含量,并对其抗氧化活性和α-葡萄糖苷酶抑制活性进行研究。结果表明,丹凤和香玉牡丹花的游离酚的总酚和总黄酮含量最高,总酚含量分别为31.45、32.64 mg/g,总黄酮含量分别为41.11、40.67 mg/g,其次是酯键合态酚和糖苷键合态酚,不溶性结合态酚含量较低;两种牡丹花游离酚均含有17种成分,其主要成分是白藜芦醇、1,2,3,4,6-O-五没食子酰葡萄糖和大波斯菊苷;酯键合态酚、糖苷键合态酚中主要成分是山柰酚-7-O-β-D-葡萄糖苷和大波斯菊苷。不同形态酚类化合物中游离酚的抗氧化活性最强,丹凤、香玉牡丹花游离酚对1,1-二苯基-2-三硝基苯肼自由基、2,2′-联氮-二(3-乙基苯并噻唑-6-磺酸)阳离子自由基清除能力和铁离子还原能力分别为855.03、367.10、230.54μmol/g和499.06、290.64、196.39μmol/g;丹凤和香玉牡丹花的游离酚对α-葡萄糖苷酶抑制活性最强,半抑制浓度分别为12.15、13.87μg/mL。研究结果对丹凤和香玉多酚利用具有一定的参考价值。

Free phenolics(FP),esterified phenolics(EP),glycosidized phenolics(GP)and insoluble bound phenolics(IBP)were successively extracted from two varieties of peony flowers:Danfeng and Xiangyu.The contents of total phenolics,total flavonoids and the major components in different phenolics fractions were determined,and their antioxidant activity andα-glucosidase inhibitory activity were also studied.It was found that for the two varieties,the contents of total phenols(31.45 and 32.64 mg/g,respectively)and total flavonoids(41.11 and 40.67 mg/g,respectively)in FP were both the highest,followed by EP and GP,and the lower values were found in IBP.Each of the two FPs contained 17 components,with the major ones being resveratrol,1,2,3,4,6-O-pentagalloylglucose and apigetrin.The major components of EP and GP were kaempferol-7-O-β-D-glucoside and apigetrin.The antioxidant activity of FP was the strongest among the four phenolics fractions.The 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical scavenging capacity and ferric reducing power of FP were 855.03,367.10,and 230.54µmol/g for Danfeng,and 499.06,290.64,and 196.39µmol/g for Xiangyu,respectively.For both varieties,FP exhibited the strongest inhibitory activity againstα-glucosidase with half maximal inhibitory concentrations of 12.15 and 13.87μg/mL,respectively.The research results are of reference significance for the utilization of polyphenols in Danfeng and Xiangyu.