基于二氧化铈/羟基氧化铁纳米酶的比色分析法快速检测泡椒凤爪中过氧化氢

Colorimetric Method Based on Cerium Dioxide/Iron Oxyhydroxide Nanozyme for Rapid Detection of Hydrogen Peroxide in Chicken Feet with Pickled Pepper

采用水热法合成二氧化铈(CeO_(2))修饰的羟基氧化铁(FeOOH),得到纳米材料CeO_(2)/FeOOH。CeO_(2)/FeOOH具有类过氧化物酶活性,可以催化过氧化氢(H_(2)O_(2))分解产生羟自由基(·OH)和超氧阴离子自由基(O2-·),将3,3’,5,5’-四甲基联苯胺(3,3’,5,5’-tetramethylbenzidine,TMB)氧化为蓝色的氧化TMB(oxidized TMB,ox-TMB)。基于此,构建基于CeO_(2)/FeOOH纳米酶的H_(2)O_(2)比色传感器。结果表明:该比色传感器对H_(2)O_(2)显示出高灵敏度响应,检测限为3.92μmol/L,检测范围为10~1000μmol/L。此外,该传感器可用于泡椒凤爪中H_(2)O_(2)的快速检测,其中,H_(2)O_(2)加标回收率为90.59%~93.42%,相对标准偏差≤4.25%,具有灵敏、准确、快速和简便等优势。

In this study,an innovative cerium dioxide-modified iron oxyhydroxide(CeO_(2)/FeOOH)nanozyme with peroxidase-like activity was synthesized by one-step hydrothermal method.The CeO_(2)/FeOOH nanozyme could catalyze the decomposition of hydrogen peroxide(H_(2)O_(2))to produce hydroxyl and superoxide anion radicals,which in turn could oxidize 3,3’,5,5’-tetramethylbenzidine(TMB)to produce a blue substance(oxidized TMB,ox-TMB).Based on this,a CeO_(2)/FeOOH-based colorimetric sensor was constructed for the detection of H_(2)O_(2).The results showed that the colorimetric sensor exhibited a highly sensitive response to H_(2)O_(2) with a detection limit of 3.92μmol/L and a linear range of 10 to 1000μmol/L.The recoveries for spiked samples ranged from 90.59%to 93.42%with relative standard deviations(RSDs)not more than 4.25%.The developed method had the advantages of high sensitivity,high accuracy,speediness and simplicity.