基于微流控芯片的鼠伤寒沙门氏菌免疫磁分离

Immunomagnetic Separation of Salmonella Typhimurium Based on Microfluidic Chip

开发一种用于鼠伤寒沙门氏菌快速分离与富集的免疫磁分离微流控系统,该系统由微流控芯片、微控制器、电磁分离与混合模块组成,具备电磁驱动混合和磁分离功能,能够实现免疫磁珠与鼠伤寒沙门氏菌的快速孵育、分离与富集。在最优条件下,可以在13 min内实现对牛奶样品中浓度范围为2×10^(1)~2×10^(6) CFU/m L鼠伤寒沙门氏菌的快速捕获与分离,捕获率在33.3%~67.5%之间,最低检测限为20 CFU/m L。因此,这种高度集成的免疫磁分离微流控系统能够从复杂食品基质中迅速、准确地富集目标细菌,为食源性致病菌的快速检测提供了有效的解决方案,对于应对食源性疾病引发的公共卫生问题具有重要意义。

This study developed an immunomagnetic separation microfluidic system for rapid isolation and enrichment of Salmonella Typhimurium.The system consisted of a microfluidic chip,a microcontroller,and an electromagnetic separation and mixing module,which had the functions of electromagnetically driven mixing and magnetic separation.It enabled rapid incubation,isolation and enrichment of immunomagnetic beads with Salmonella Typhimurium.Under the optimal conditions,the rapid capture and separation of Salmonella Typhimurium in milk samples in the concentration range from 2×10^(1) to 2×10^(6) CFU/mL were achieved within 13 min,with capture rates between 33.3%and 67.5%.The limit of detection was 20 CFU/mL.This highly integrated immunomagnetic separation microfluidic system can enrich target bacteria from complex food matrices rapidly and accurately,thus providing an effective solution for the rapid detection of foodborne pathogenic bacteria,which is of great significance in addressing public health problems caused by foodborne diseases.