流动注射法测定海水中挥发性酚的方法研究

基于HJ 825—2017《水质挥发性酚的测定流动注射-4-氨基安替比林分光光度法》中未规定海水挥发性酚的测定及传统国标法GB 17378.4—2007《海洋监测规范第4部分:海水分析》操作繁琐、效率低及精度差等不足,通过海水盐度基体干扰消除、试剂条件优化、仪器参数优化及方法性能指标的测试,建立了测定海水挥发性酚的流动注射分析方法。实验结果表明,该分析方法的测定范围为0.00~100.00μg/L,相关系数为0.9999,检出限为0.44μg/L,测定下限为1.76μg/L,加标样品的回收率为96.9%~101.0%,相对标准偏差为0.34%~2.57%,有证标准物质的相对误差范围为-2.30%~-0.09%。本方法的线性系数、正确度、精密度及检出限均满足GB 3097—1997《海水水质标准》的要求,能满足大批量样品分析的工作需要。

Based on the lack of determination of volatile phenols in seawater by Water Quality-Determination of volatile phenols-Flow injection analysis(FIA)and 4-AAP spectrophotometric method(HJ 825-2017)and the shortcomings of the traditional national standard method of the Specification for marine monitoring—Part 4:Seawater analysis(GB 17378.4-2007),such as cumbersome operation,low efficiency and poor accuracy,a flow injection analysis method for the determination of volatile phenols in seawater was established by eliminating the interference of seawater salinity matrix,optimizing reagent conditions,optimizing instrument parameters and testing method performance indicators.The experimental results showed that the determination range of the analytical method was 0.00~100.00μg/L,the correlation coefficient was 0.9999,the detection limit was 0.44μg/L,the lower limit of determination was 1.76μg/L,the recoveries of the samples ranged from 96.9%to 101.0%,the RSD(n=6)was 0.34%-2.57%,and the relative error range of the certified reference material was-2.30%--0.09%.The linearity,accuracy,precision and detection limit of the method all met the requirements of the Standard for Seawater Quality(GB 3097-1997)and could meet the needs of large quantities of sample analysis.