振腹推拿手法对抽动障碍模型大鼠纹状体及皮质区神经营养因子水平的影响

The effect of abdominal massage therapy on the levels of neurotrophic factors in the striatum and cortex of tic disorder model rats

目的探讨振腹推拿手法对亚氨基二丙腈诱导的抽动障碍大鼠模型的纹状体及皮质区神经营养因子水平的影响。方法将40只4周龄SD大鼠随机分为空白组10只、模型组10只、推拿组10只、盐酸硫必利组10只。各造模组予腹腔注射亚氨基二丙腈[300 mg/kg,10 mL/(kg·d)],空白组予等体积生理盐水腹腔注射,连续7天。造模成功后进行干预,空白组和模型组予生理盐水灌胃,盐酸硫必利组予硫必利混悬液灌胃(1 mL药液中约含2.1 mg原药),3组大鼠灌胃体积均为1 mL/100 g,推拿组在神阙穴行振腹推拿手法操作,灌胃及振腹操作均每日1次,连续5周。末次干预完成后,采用ELISA法检测大鼠纹状体及皮质区脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、胶质细胞源性神经营养因子(glial cell derived neurotrophic factor,GDNF)、血管内皮生长因子(vascular endothelial growth factor,VEGF)和神经生长因子(nerve growth factor,NGF)水平。结果与空白组比较,模型组纹状体区BDNF、GDNF、VEGF、NGF水平均显著降低(P<0.01),皮质区BDNF、VEGF、NGF显著降低(P<0.01),GDNF水平降低(P<0.05);与模型组比较,盐酸硫必利组纹状体区4种因子水平均显著升高(P<0.01),皮质区BDNF、VEGF、NGF显著升高(P<0.01);与模型组比较,推拿组纹状体区GDNF、NGF显著升高(P<0.01),皮质区BDNF升高(P<0.05),VEGF、NGF均显著升高(P<0.01)。结论振腹推拿手法可提高抽动障碍大鼠纹状体及皮质区神经营养因子的水平。

Objective To investigate the effect of abdominal massage therapy on the levels of neurotrophic factors in the striatum and cortex of rats with tic disorder induced by IDPN.Methods 40 SD rats aged 4 weeks were randomly divided into the control group,the model group,the massage group,and the tiapride hydrochloride group,with 10 rats in each group.Each modeling group was given intraperitoneal injection of IDPN[300 mg/kg,10 mL/(kg•d)],while the control group was given intraperitoneal injection of equal volume physiological saline for 7 consecutive days.After successful modeling,the control group and the model group were given physiological saline by gavage,while the tiapride hydrochloride group was given a suspension of tiapride by gavage(1 mL of the drug contains approximately 2.1 mg of the original drug),with a gavage volume of 1 mL/100 g;the massage group was given abdominal massage therapy on Shenque acupoint,with gavage and massage once a day for 5 consecutive weeks.After the final intervention,the levels of brain-derived neurotrophic factor(BDNF),glial cell derived neurotrophic factor(GDNF),vascular endothelial growth factor(VEGF)and nerve growth factor(NGF)in striatum and cortex of rats were detected by ELISA.Results Compared with the control group,the levels of BDNF,GDNF,VEGF and NGF in striatum area were significantly decreased in the model group(P<0.01),and the levels of BDNF,VEGF,and NGF in the cortex area were significantly decreased(P<0.01),and the level of GDNF was decreased(P<0.05).Compared with model group,the levels of 4 factors in striatum area were significantly increased in the tiapride hydrochloride group(P<0.01),and the levels of BDNF,VEGF and NGF in cortex area were significantly increased(P<0.01).Compared with model group,GDNF and NGF in striatum area were significantly increased in the massage group(P<0.01),and BDNF in cortex area were increased(P<0.05),VEGF and NGF in cortex area were significantly increased(P<0.01).Conclusion Abdominal massage can improve the levels of neurotrophic factors in striatum and cortex of rats with tic disorder.