突发性耳聋病人血清Cx43、GABA水平及其与听力功能的相关性

Serum Cx43 and GABA levels in patients with sudden deafness and their correlation with hearing function

目的:探讨γ-氨基丁酸(GABA)、连接蛋白43(Cx43)在突发性耳聋病人中的表达及与听力功能的相关性。方法:选取100例突发性耳聋病人为观察组,根据年龄、性别随机选择100名健康体检者为对照组。收集一般资料,采用酶联免疫吸附法检测血清中GABA、Cx43表达水平,Pearson法分析GABA、Cx43表达的相关性,ROC曲线评价GABA、Cx43表达水平对突发性耳聋的诊断价值。采用logistic回归分析影响突发性耳聋病人听力功能的因素。结果:与对照组相比,观察组病人血清中Cx43、GABA表达水平均明显下降(P<0.01),且听力功能异常病人血清中Cx43、GABA表达水平明显低于听力功能正常病人(P<0.01);突发性耳聋病人血清中GABA与Cx43表达呈正相关关系(r=0.472,P<0.05);ROC曲线结果显示,血清GABA、Cx43水平诊断突发性耳聋的AUC分别为0.938、0.900,对应的敏感度分别为86.00%、87.00%,特异度分别为92.00%、79.00%,二者联合诊断的AUC为0.963,敏感度为91.00%,特异度为87.00%。logistic回归分析结果显示GABA、Cx43均是突发性耳聋病人听力功能的独立影响因素(P<0.01)。结论:GABA、Cx43在突发性耳聋病人血清中表达水平均降低,与病人听力功能密切相关,联合检测二者可作为诊断突发性耳聋及听力功能异常的生物学指标。

Objective:To investigate the expression ofγ-aminobutyric acid(GABA)and connexin 43(Cx43)in patients with sudden deafness and their correlation with hearing function.Methods:A total of 100 patients with sudden deafness 2019 to were selected as the observation group.According to age and gender,100 healthy subjects were randomly selected as the control group.The general information was collected,the expression levels of GABA and Cx43 in serum were detected by enzyme-linked immunosorbent assay.Pearson method was used to analyze the correlation of GABA and Cx43 expression,and ROC curve was used to evaluate the diagnostic value of GABA and Cx43 expression levels for sudden deafness.Logistic regression analysis was used to analyze the factors affecting hearing function in patients with sudden deafness.Results:Compared with the control group,the expression levels of serum Cx43 and GABA in the serum of the patients in the observation group were decreased(P<0.01),and the expression levels of Cx43 and GABA in the serum of the patients with abnormal hearing function were lower than those of the patients with normal hearing function(P<0.01).The expression of GABA in serum of patients with sudden deafness was positively correlated with Cx43(r=0.472,P<0.05).The ROC curve results showed that the AUC of serum GABA and Cx43 levels for the diagnosis of sudden deafness was 0.938 and 0.900,respectively,the corresponding sensitivity was 86.00%and 87.00%,and the specificity was 92.00%and 79.00%,respectively,the AUC of the combined diagnosis was 0.963,the sensitivity was 91.00%,and the specificity was 87.00%.Logistic regression analysis showed that GABA and Cx43 were independent influencing factors of hearing function in patients with sudden deafness(P<0.01).Conclusions:The expression levels of GABA and Cx43 in the serum of patients with sudden deafness are decreased,which are closely related to the hearing function of the patients.The combined detection of the two can be used as biological indicators for the diagnosis of sudden deafness and abnormal hearing function.