HPLC法测定麻杏石甘口服液中甘草酸含量

Determination of glycyrrhizic acid in Maxing Shigan oral liquid by HPLC

试验旨在建立1种高效液相色谱法(HPLC)测定麻杏石甘口服液中甘草酸含量的分析方法。样品经流动相提取、过滤,注入高效液相色谱仪进行检测。色谱柱为Agilent ZORBAX SB-C18 (4.6 mm×250 mm,5μm),以甲醇-0.2 mol/L乙酸铵溶液-冰乙酸(体积比为67∶33∶1)为流动相,检测波长为250 nm,流速1.0 mL/min,柱温30℃,进样量10μL。结果表明,该方法在0.1~1.0 g/L范围内线性良好,甘草酸含量的线性方程为Y=7 767.13X-63.247,r=0.999 8。该方法具有较好的精密度、准确度和重复性,加样回收率为92.86%~96.19%,相对标准偏差值(RSD)均小于2.0%,适用于测定麻杏石甘口服液中甘草酸含量。

The aim of this study was to establish a method for the determination of glycyrrhizic acid in Maxing Shigan oral liquid by high performance liquid chromatography(HPLC).The samples were extracted by mobile phase,filtered and injected into high performance liquid chromatography for detection.The determination was performed on Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)column with methanol-0.2 mol/L ammonium acetate solution-glacial acetic acid(the volume ratio was 67∶33∶1)as the mobile phase.The detection wavelength was set at 250 nm,the flow rate was 1.0 mL/min,and the column temperature was 30℃,the sample size was 10μL.The results show that the method has good linearity in the range of 0.1~1.0 g/L,and the linear equation of glycyrrhizic acid was Y=7767.13X-63.247,r=0.9998.The method has good precision,accuracy and repeatability.The recoveries were 92.86%~96.19%,and the relative standard deviations(RSD)are less than 2.0%.It is suitable for the determination of glycyrrhizic acid in Maxing Shigan oral liquid.